Tourtellotte W W, Ma B I, Brandes D B, Walsh M J, Potvin A R
Ann Neurol. 1981 Jun;9(6):551-6. doi: 10.1002/ana.410090607.
De novo central nervous system (CNS) IgG measles antibody synthesis rate was determined in two patients with subacute sclerosing panencephalitis (SSPE). Cerebrospinal fluid and sera were incubated with different concentrations of measles virus to determine maximum absorption of IgG. The difference in IgG concentration before and after incubation was considered the amount of measles-specific IgG antibody. Various tests were done to assess the immunological specificity of the absorption test. Using our empirical formula, the de novo CNS IgG measles antibody synthesis rate for one patient with SSPE was 57 mg/day, or 59% of the total CNS IgG synthesis rate; for the other it was 145 mg/day, or 82%. Daily determination of CNS measles antibody by empirical formula provides a quantification of humoral immunity in SSPE that corrects for both passage of measles antibody across a damaged blood-brain barrier and the effect of an elevated blood antibody level.
测定了两名亚急性硬化性全脑炎（SSPE）患者中枢神经系统（CNS）IgG麻疹抗体的从头合成率。将脑脊液和血清与不同浓度的麻疹病毒孵育，以确定IgG的最大吸收量。孵育前后IgG浓度的差异被视为麻疹特异性IgG抗体的量。进行了各种测试以评估吸收试验的免疫特异性。使用我们的经验公式，一名SSPE患者的中枢神经系统IgG麻疹抗体从头合成率为57毫克/天，占中枢神经系统IgG总合成率的59%；另一名患者为145毫克/天，占82%。通过经验公式每日测定中枢神经系统麻疹抗体可对SSPE中的体液免疫进行量化，该量化校正了麻疹抗体穿过受损血脑屏障的情况以及血液抗体水平升高的影响。
