Reiner R G, Tanner A R, Keyhani A H, Wright R
Clin Exp Immunol. 1981 Feb;43(2):376-80.
Macrophages have been isolated and cultured in vitro from normal rat livers and from livers into which macrophages have been recruited in vivo, following an intravenous injection of killed Corynebacterium parvum. Simultaneously, peripheral blood monocytes have been isolated and cultured in vitro. After 24 hr in culture, supernatants and cell lysates were harvested and the activity of a lysosomal enzyme, N-acetyl-glucosaminidase (NAG), measured. NAG activity in the cell lysates of the recruited tissue macrophages was significantly higher than that measured in control tissue macrophages. Increased NAG activity was also observed in the supernatants from the recruited macrophages. In contrast, the NAG activity in cell lysates and supernatants of peripheral monocytes was not significantly changed after C. parvum injection. In this animal model, measurement of a lysosomal enzyme produced by peripheral monocytes did not reflect the magnitude of the changes observed for the tissue macrophages.
已从正常大鼠肝脏以及静脉注射灭活微小棒状杆菌后在体内募集了巨噬细胞的肝脏中分离并体外培养巨噬细胞。同时，已分离并体外培养外周血单核细胞。培养24小时后，收集上清液和细胞裂解物，并测量溶酶体酶N-乙酰葡糖胺酶（NAG）的活性。募集的组织巨噬细胞的细胞裂解物中的NAG活性显著高于对照组织巨噬细胞中测得的活性。在募集的巨噬细胞的上清液中也观察到NAG活性增加。相比之下，微小棒状杆菌注射后外周单核细胞的细胞裂解物和上清液中的NAG活性没有显著变化。在这个动物模型中，外周单核细胞产生的溶酶体酶的测量并未反映出组织巨噬细胞所观察到的变化程度。
