Gittzus J G, Rubin S J
J Clin Microbiol. 1977 Dec;6(6):574-7. doi: 10.1128/jcm.6.6.574-577.1977.
Commercially prepared antibody conjugates that are now available allow extensive application of direct immunofluorescence for diagnosing herpetic infections. Five of these conjugates--Microbiological Associates bivalent herpes antiserum (MBA-bivalent), MBA anti-herpes types 1 (MBA-1) and 2 (MBA-2), and Flow Laboratories anti-herpes types 1 (Flow-1) and 2 (Flow-2)--were compared for antibody titer, diagnostic accuracy, and nonspecific background staining on known infected tissue. Four of these were tested simultaneously on 75 clinical specimens. No attempt was made to differentiate between herpes simplex virus type 1 or type 2 infections. All antisera were comparable in titer and exhibited fluorescence with both herpes simplex virus types 1 and 2 antigens. The most significant difference was excessive nonspecific staining consistently seen with the MBA-2 conjugate. Overall, Flow-2 and MBA-bivalent antisera were preferable.
目前市售的商业化制备抗体偶联物使得直接免疫荧光法在诊断疱疹感染方面得到了广泛应用。对其中五种偶联物——微生物协会双价疱疹抗血清（MBA - 双价）、MBA抗1型疱疹（MBA - 1）和抗2型疱疹（MBA - 2），以及福罗实验室抗1型疱疹（Flow - 1）和抗2型疱疹（Flow - 2）——进行了抗体效价、诊断准确性以及在已知感染组织上的非特异性背景染色方面的比较。其中四种同时在75份临床标本上进行了检测。未尝试区分1型单纯疱疹病毒或2型单纯疱疹病毒感染。所有抗血清的效价相当，并且对1型和2型单纯疱疹病毒抗原均呈现荧光。最显著的差异是MBA - 2偶联物始终出现过度的非特异性染色。总体而言，Flow - 2和MBA - 双价抗血清更具优势。
