用于厌氧血症快速初步诊断的阳性血培养物的气液色谱法。
Gas-liquid chromatography of positive blood cultures for rapid presumptive diagnosis of anaerobic bacteremia.
作者信息
Edson R S, Rosenblatt J E, Washington J A, Stewart J B
出版信息
J Clin Microbiol. 1982 Jun;15(6):1059-61. doi: 10.1128/jcm.15.6.1059-1061.1982.
Abstract
Gas-liquid chromatographic analysis of volatile fatty acid patterns was performed when growth was initially detected in 87 positive blood cultures. Isovaleric acid, butyric acid, or both were found in 39 of 43 (90%) blood cultures that contained anaerobes but were absent in all 44 cultures that contained facultatively anaerobic or aerobic bacteria. The detection of isovaleric acid indicated the presence of Bacteroides fragilis, as this acid was found in 88% of unimicrobial and 75% of polymicrobial bacteremias due to B. fragilis. Butyric acid was detected most often in blood cultures which grew Fusobacterium spp. (three of three), Clostridium spp. (six of seven), or both (three of four).
摘要
当在87份阳性血培养物中最初检测到生长时，进行了挥发性脂肪酸模式的气-液色谱分析。在43份含有厌氧菌的血培养物中的39份（90%）中发现了异戊酸、丁酸或两者都有，但在所有44份含有兼性厌氧或需氧菌的培养物中均未发现。异戊酸的检测表明存在脆弱拟杆菌，因为在88%的单一微生物血症和75%的由脆弱拟杆菌引起的多微生物血症中发现了这种酸。丁酸最常在生长梭杆菌属（3份中的3份）、梭菌属（7份中的6份）或两者都生长（4份中的3份）的血培养物中检测到。
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本文引用的文献
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2
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3
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