Goycoolea M V, Paparella M M, Goldberg B, Carpenter A M
Arch Otolaryngol. 1980 Jul;106(7):430-3. doi: 10.1001/archotol.1980.00790310054014.
A round window membrane permeability study of macromolecules in otitis media, using tritiated normal human serum albumin, was done in cats. Eustachian tube obstruction was used to induce otitis media in ten animals. Samples of perilymph in obstructed and normal control ears were studied after 25 minutes of exposure of the round window to tritiated albumin. Twelve additional animals were obstructed, and their round window membranes studied to provide histopathologic evidence for our permeability studies. In this first report of round windon membrane permeability to macromolecules in otitis media, we observed an increased permeability to tritiated albumin in diseased ears. Evidence suggests a pathophysiological explanation for the association of otitis media and sensorineural hearing loss and/or endolymphatic hydrops. Proof that large molecules pass suggests the feasibility of diffusion of smaller molecules (toxins and enzymes) through the round window membrane.
利用氚标记的正常人血清白蛋白，在猫身上进行了一项关于中耳炎中大分子物质圆窗膜通透性的研究。通过咽鼓管阻塞法在10只动物身上诱发中耳炎。在圆窗暴露于氚标记白蛋白25分钟后，对阻塞耳和正常对照耳的外淋巴样本进行了研究。另外12只动物被阻塞咽鼓管，并对其圆窗膜进行研究，为我们的通透性研究提供组织病理学证据。在这份关于中耳炎中圆窗膜对大分子物质通透性的首次报告中，我们观察到患病耳对氚标记白蛋白的通透性增加。有证据表明，中耳炎与感音神经性听力损失和/或内淋巴积水之间的关联存在病理生理学解释。大分子能够通过的证据表明小分子（毒素和酶）通过圆窗膜扩散是可行的。
