Mathieson R A, Kitts W D
J Endocrinol. 1980 May;85(2):317-25. doi: 10.1677/joe.0.0850317.
The binding of the phyto-oestrogens, genistein and coumestrol by oestrogen receptors in pituitary and hypothalamic cytosol of the ewe was studied in relation to the binding of [3H]-oestradiol-17 beta. It was determined that the interaction of these compounds was competitive. The apparent dissociation constant for oestradiol-17 beta in ewe pituitary cytosol was determined to be 0 x 26 +/- 0 x 12 (S.E.M.) nmol/l. Apparent inhibition constants for coumestrol and genistein were determined to be 60 and 170 nmol/l respectively. These results suggested that phyto-oestrogens can interfere with the normal oestrogen feedback mechanisms with respect to release of gonadotrophine in the ewe.
研究了植物雌激素染料木黄酮和香豆雌酚与母羊垂体和下丘脑细胞质中雌激素受体的结合情况，并与[3H]-雌二醇-17β的结合进行了比较。结果表明，这些化合物之间的相互作用具有竞争性。母羊垂体细胞质中雌二醇-17β的表观解离常数为0×26±0×12（标准误）nmol/l。香豆雌酚和染料木黄酮的表观抑制常数分别为60和170 nmol/l。这些结果表明，植物雌激素可能会干扰母羊促性腺激素释放方面的正常雌激素反馈机制。
