脱氧寡核苷酸固相磷三酯合成法的改进
Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.
作者信息
Patel T P, Millican T A, Bose C C, Titmas R C, Mock G A, Eaton M A
出版信息
Nucleic Acids Res. 1982 Sep 25;10(18):5605-20. doi: 10.1093/nar/10.18.5605.
Abstract
A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed with other reagents such as trichloroacetic acid. Coupling reactions are described using above ambient temperatures with no detectable increase in side products. Both procedures have been successfully incorporated into an automated system, which can compete with the rate of synthesis by the phosphite approach.
摘要
苯磺酸在二甲基甲酰胺和二氯甲烷混合液（9:1，体积比）中的3%（重量/体积）溶液被证明是一种用于使连接在固相载体上的脱氧寡核苷酸脱三苯甲基的非常有效的试剂。该试剂的脱嘌呤水平低于用三氯乙酸等其他试剂时观察到的水平。描述了在高于环境温度下进行的偶联反应，副产物没有可检测到的增加。这两种方法都已成功地纳入自动化系统，该系统在合成速率上可与亚磷酸酯法相媲美。
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本文引用的文献
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DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
2
Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.多核苷酸的固相合成。IV. 磷酸三酯法中聚苯乙烯树脂在聚脱氧核糖核苷酸合成中的应用。
Nucleic Acids Res. 1980 Nov 25;8(22):5507-17. doi: 10.1093/nar/8.22.5507.
3
Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.多核苷酸的固相合成。III. 用片段偶联磷酸三酯法合成具有特定序列的多核苷酸。
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Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.多核苷酸的固相合成。II. 用片段偶联磷酸三酯法合成聚胸腺嘧啶核苷酸
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