Blunden R W, Farley J, Geary T D, Halliday J W, Hawley D, Rudzki Z
Pathology. 1980 Oct;12(4):575-85. doi: 10.3109/00313028009086810.
Two surveys of the proficiency of performance of serum ferritin estimation in Australia have been conducted, one in 1978 and one in 1979. The majority of participants in both surveys used assay kits supplied by Clinical Assays, Roche Diagnostics and Ramco. The first survey showed a median intrabatch variation 9% and median interbatch variation of 17%. The overall assay variation had a median level of 22%. Assays by the various kits gave values which were not significantly different for each specimen (including purified liver and spleen ferritin solutions). The second survey showed an improvement in the overall precision of the participants with a median coefficient of variation of 15%. Contrary to the first survey, significant differences were evident between the results in 4 of the 5 specimens assayed. Some participants returned results for the high ferritin serum that differed substantially depending on the dilution of the sample used. The surveys showed a need for improvement in the assay performance of some participants, the need for ferritin levels to be assayed at least in two dilutions, and the need for standardization of assays, reference ranges and reporting units. Recommendation are made on all these points.
澳大利亚已开展了两项关于血清铁蛋白测定操作熟练度的调查，一项于1978年进行，另一项于1979年进行。两项调查中的大多数参与者都使用了由临床检验公司、罗氏诊断公司和拉姆科公司提供的检测试剂盒。第一次调查显示批内变异中位数为9%，批间变异中位数为17%。总体检测变异的中位数水平为22%。使用各种试剂盒进行的检测，对每个样本（包括纯化的肝脏和脾脏铁蛋白溶液）得出的值没有显著差异。第二次调查显示参与者的总体精密度有所提高，变异系数中位数为15%。与第一次调查相反，在检测的5个样本中的4个样本的结果之间存在明显差异。一些参与者返回的高铁蛋白血清结果因所用样本的稀释度不同而有很大差异。调查表明，一些参与者的检测性能需要改进，铁蛋白水平至少需要在两种稀释度下进行检测，并且检测、参考范围和报告单位需要标准化。针对所有这些要点提出了建议。
