Ma J K, Hein M B
Department of Immunology, United Medical School, Guy's Hospital, London, United Kingdom.
Plant Physiol. 1995 Oct;109(2):341-6. doi: 10.1104/pp.109.2.341.
The original report of Hiatt (1989) initiated a wave of excitement at the realization that a complex mammalian protein such as immunoglobulin could be assembled within a plant cell. The general reaction was one of amazement, but interest in exploiting the possibilities arising from the discovery, for example to make antibodies of therapeutic value, has taken a considerable time to develop. In the meantime, other recombinant expression systems and traditional cell culture techniques have advanced and overcome some of their problems, particularly those associated with yields. Plants, however, still offer unique advantages, especially in their ability to match the protein assembly capabilities of mammalian cells (as demonstrated by the assembly of SIgA molecules), as well as to provide antibodies in bulk at low cost. In addition, the area of "immunization" of plants holds great promise and will surely be a field of enormous growth for the future.
海厄特（1989年）的原始报告引发了一阵热潮，因为人们意识到诸如免疫球蛋白这样的复杂哺乳动物蛋白能够在植物细胞内组装。普遍的反应是惊叹不已，但是要开发利用这一发现所带来的可能性，比如制造具有治疗价值的抗体，却花了相当长的时间。与此同时，其他重组表达系统和传统细胞培养技术取得了进展并克服了一些问题，尤其是那些与产量相关的问题。然而，植物仍然具有独特的优势，特别是在匹配哺乳动物细胞的蛋白质组装能力方面（如分泌型免疫球蛋白A分子的组装所示），以及以低成本大量生产抗体方面。此外，植物“免疫”领域前景广阔，必将成为未来一个巨大的增长领域。
