Sadeghi E, Matlow A, MacLusky I, Karmali M A
Department of Microbiology, Hospital for Sick Children, Toronto, Ontario, Canada.
J Clin Microbiol. 1994 Jan;32(1):54-8. doi: 10.1128/jcm.32.1.54-58.1994.
The utility of sputum Gram stain in assessing salivary contamination and in predicting the presence of pathogens on the basis of morphology was investigated in 287 respiratory specimens from patients with cystic fibrosis. Where acceptability for culture was defined as a leukocyte/squamous epithelial cell ratio of > 5, 76.6% (220 of 287) of respiratory specimens received in the laboratory were considered acceptable. Unacceptable specimens were more common in younger patients. The positive predictive value of the Gram stain for growth from acceptable sputum samples was 98% for Pseudomonas aeruginosa, 84.4% for Pseudomonas cepacia, 86.3% for Staphylococcus aureus, and 100% for Haemophilus influenzae. In cystic fibrosis patients, as has been reported for respiratory specimens in general, Gram stain of respiratory specimens in helpful for interpreting culture results.
在287份来自囊性纤维化患者的呼吸道标本中，研究了痰革兰氏染色在评估唾液污染以及根据形态学预测病原体存在方面的效用。若将培养的可接受性定义为白细胞/鳞状上皮细胞比率大于5，则实验室收到的呼吸道标本中有76.6%（287份中的220份）被认为是可接受的。不可接受的标本在年轻患者中更为常见。对于可接受痰标本的培养生长，革兰氏染色对铜绿假单胞菌的阳性预测值为98%，对洋葱伯克霍尔德菌为84.4%，对金黄色葡萄球菌为86.3%，对流感嗜血杆菌为100%。在囊性纤维化患者中，正如一般呼吸道标本所报道的那样，呼吸道标本的革兰氏染色有助于解释培养结果。
