Calia K E, Murtagh M, Ferraro M J, Calderwood S B
Infectious Disease Unit, Massachusetts General Hospital, Boston 02114.
Infect Immun. 1994 Apr;62(4):1504-6. doi: 10.1128/iai.62.4.1504-1506.1994.
Vibrio cholerae O139 is a recently identified non-O1 V. cholerae strain responsible for outbreaks of epidemic cholera in India, Bangladesh, and Thailand in the past 2 years. Other workers have demonstrated the presence of the cholera toxin genetic element in V. cholerae O139, unlike the situation for other non-O1 V. cholerae strains. We sought to compare further this strain with strains of V. cholerae O1, classical and El Tor biotypes, by classic microbiologic methods, Southern blot analysis for restriction fragment length polymorphisms with probes for iron-regulated genes of V. cholerae O1, and comparisons of outer membrane protein profiles. Our results were similar for V. cholerae O139 and the El Tor biotype of V. cholerae O1, with the exception of the constitutive expression in V. cholerae O139 of OmpS, an outer membrane protein that was maltose inducible in comparison strains of V. cholerae O1.
霍乱弧菌O139是最近发现的一种非O1霍乱弧菌菌株，在过去两年里它在印度、孟加拉国和泰国引发了霍乱疫情。与其他非O1霍乱弧菌菌株的情况不同，其他研究人员已证明霍乱弧菌O139中存在霍乱毒素遗传元件。我们试图通过经典微生物学方法、用霍乱弧菌O1铁调节基因探针进行的限制性片段长度多态性的Southern印迹分析以及外膜蛋白谱比较，进一步将该菌株与霍乱弧菌O1的菌株（古典生物型和埃尔托生物型）进行比较。除了霍乱弧菌O139中OmpS（一种外膜蛋白）的组成型表达外，我们得到的结果在霍乱弧菌O139和霍乱弧菌O1的埃尔托生物型之间是相似的，在比较的霍乱弧菌O1菌株中，该外膜蛋白是麦芽糖诱导型的。
