Lopategui J R, Gaffey M J, Frierson H F, Chan J K, Mills S E, Chang K L, Chen Y Y, Weiss L M
Department of Pathology, City of Hope National Medical Center, Duarte, California 91010.
Am J Surg Pathol. 1994 Apr;18(4):391-8. doi: 10.1097/00000478-199404000-00007.
Undifferentiated carcinoma of the nasopharynx has a well-known association with Epstein-Barr virus (EBV), but only an inconsistent relationship has been identified in undifferentiated carcinomas occurring at other sites. We investigated 22 formalin-fixed, paraffin-embedded cases of sinonasal undifferentiated carcinomas (SNUCs) occurring in Western and Asian patients. A highly sensitive in situ hybridization method was performed using an antisense oligonucleotide probe to the EBER1 gene of EBV. We identified EBV RNA in seven of 11 SNUCs from Asian patients, but in none of the Western SNUC patients (0/11). The EBER1 signal was present in all or virtually all of the tumor cell nuclei in the seven EBV-RNA-positive Asian SNUCs. The latent membrane protein-1 (LMP1) of EBV was not identified in any of the five positive cases tested. Our results suggest that genetic predisposition or environmental/geographical cofactors play an important role in determining the strength of the association of SNUC with EBV.
鼻咽未分化癌与爱泼斯坦-巴尔病毒（EBV）有着众所周知的关联，但在其他部位发生的未分化癌中，仅发现了一种不连贯的关系。我们研究了22例发生在西方和亚洲患者中的经福尔马林固定、石蜡包埋的鼻窦未分化癌（SNUC）病例。使用针对EBV的EBER1基因的反义寡核苷酸探针进行了一种高度敏感的原位杂交方法。我们在11例亚洲患者的SNUC中发现了7例EBV RNA，但在西方SNUC患者中均未发现（0/11）。在7例EBV-RNA阳性的亚洲SNUC中，所有或几乎所有肿瘤细胞核中都存在EBER1信号。在所检测的5例阳性病例中，均未发现EBV的潜伏膜蛋白1（LMP1）。我们的结果表明，遗传易感性或环境/地理辅助因素在确定SNUC与EBV关联的强度方面起着重要作用。
