Howlett C R, Evans M D, Walsh W R, Johnson G, Steele J G
School of Pathology, University of New South Wales, Kensington, Australia.
Biomaterials. 1994 Feb;15(3):213-22. doi: 10.1016/0142-9612(94)90070-1.
The suitability of polymeric biomaterials as surfaces for the attachment and growth of cells has often been investigated in cell culture. In this study the contribution that serum fibronectin (Fn) or vitronectin (Vn) make to the attachment and spreading of cells cultured from explanted human bone (bone-derived cells) during the first 90 min of culture was determined for metallic and ceramic surfaces. The requirement for Fn or Vn for attachment and spreading of bone-derived cells onto stainless steel 316 (SS), titanium (Ti) and alumina (Al2O3) and to polyethyleneterephthalate (PET) was directly tested by selective removal of Fn or Vn from the serum prior to addition to the culture medium. Attachment and spreading of bone-derived cells onto SS, Ti and Al2O3 surfaces were reduced by 73-83% when the cells were seeded in medium containing serum from which the Vn had been removed. Cell attachment and spreading on these surfaces when seeded in medium containing Fn-depleted serum (which contained Vn) were not reduced to the same extent as in the medium containing Vn-depleted serum. The bone-derived cells failed to attach to the surfaces to the same extent when seeded in medium containing serum depleted of both Vn and Fn. Our results show that for human bone-derived cells, the attachment and spreading of cells onto SS, Ti and Al2O3 as well as PET during the first 90 min of a cell culture attachment assay are a function of adsorption of serum Vn onto the surface.
在细胞培养中，人们经常研究聚合生物材料作为细胞附着和生长表面的适用性。在本研究中，测定了血清纤连蛋白（Fn）或玻连蛋白（Vn）对从人骨外植体培养的细胞（骨源细胞）在培养最初90分钟内附着和铺展的贡献，研究对象为金属和陶瓷表面。通过在添加到培养基之前从血清中选择性去除Fn或Vn，直接测试了骨源细胞附着和铺展到不锈钢316（SS）、钛（Ti）、氧化铝（Al2O3）以及聚对苯二甲酸乙二酯（PET）表面对Fn或Vn的需求。当细胞接种在去除了Vn的血清培养基中时，骨源细胞在SS、Ti和Al2O3表面的附着和铺展减少了73 - 83%。当细胞接种在含有去除了Fn的血清（含有Vn）的培养基中时，这些表面上的细胞附着和铺展没有像在含有去除了Vn的血清的培养基中那样减少到相同程度。当细胞接种在同时去除了Vn和Fn的血清培养基中时，骨源细胞在这些表面上的附着程度降低。我们的结果表明，对于人骨源细胞，在细胞培养附着试验的最初90分钟内，细胞在SS、Ti、Al2O3以及PET表面的附着和铺展是血清Vn吸附到表面的函数。
