Raskin L, Poulsen L K, Noguera D R, Rittmann B E, Stahl D A
Department of Civil Engineering, University of Illinois at Urbana-Champaign, 61801.
Appl Environ Microbiol. 1994 Apr;60(4):1241-8. doi: 10.1128/aem.60.4.1241-1248.1994.
The microbial community structure of anaerobic biological reactors was evaluated by using oligonucleotide probes complementary to conserved tracts of the 16S rRNAs of phylogenetically defined groups of methanogens. Phylogenetically defined groups of methanogens were quantified and visualized, respectively, by hybridization of 32P- and fluorescent-dye-labeled probes to the 16S rRNAs from samples taken from laboratory acetate-fed chemostats, laboratory municipal solid waste digestors, and full-scale sewage sludge digestors. Methanosarcina species, members of the order Methanobacteriales, and Methanosaeta species were the most abundant methanogens present in the chemostats, the solid-waste digestors, and the sewage sludge digestors, respectively.
通过使用与系统发育定义的产甲烷菌群体的16S rRNA保守区域互补的寡核苷酸探针，对厌氧生物反应器的微生物群落结构进行了评估。通过将32P标记和荧光染料标记的探针与取自实验室乙酸盐喂养的恒化器、实验室城市固体废物消化器和全规模污水污泥消化器的样品中的16S rRNA杂交，分别对系统发育定义的产甲烷菌群体进行了定量和可视化。甲烷八叠球菌属物种、甲烷杆菌目成员和甲烷丝状菌属物种分别是恒化器、固体废物消化器和污水污泥消化器中最丰富的产甲烷菌。
