用于腺病毒表征的定量比色微中和试验
Quantitative colorimetric microneutralization assay for characterization of adenoviruses.
作者信息
Crawford-Miksza L K, Schnurr D P
机构信息
Viral and Rickettsial Disease Laboratory, California Department of Health Services, Berkeley 94704.
出版信息
J Clin Microbiol. 1994 Sep;32(9):2331-4. doi: 10.1128/jcm.32.9.2331-2334.1994.
Abstract
A microneutralization assay that automates the reading and interpretation of viral infectivity and neutralization data was developed for the characterization of adenoviruses (AV). Virus, serum, and cells were added to 96-well plates, incubated for 7 days, and stained with vital stain. The A550 of solubilized dye was read in a plate reader interfaced to a personal computer which analyzed the results. Correlation of A550 values with visual observation of cytopathic effect was extremely good (r = 0.977625). Clinical isolates of 17 AV from 11 patients were characterized by colorimetric neutralization assay. Prototype AV titers tested were comparable to those determined by tube methods. Prototype homotypic antiserum titers were comparable to or greater than those determined by standard tube neutralization.
摘要
开发了一种微中和试验，该试验可自动读取和解释病毒感染性及中和数据，用于腺病毒（AV）的特性鉴定。将病毒、血清和细胞加入96孔板中，孵育7天，并用活性染料染色。在与个人计算机相连的酶标仪中读取溶解染料的A550值，由计算机分析结果。A550值与细胞病变效应的视觉观察结果相关性非常好（r = 0.977625）。通过比色中和试验对11例患者的17株腺病毒临床分离株进行了特性鉴定。测试的原型腺病毒滴度与通过试管法测定的滴度相当。原型同型抗血清滴度与标准试管中和法测定的滴度相当或更高。
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