Fife K H, Ashley R, Shields A F, Salter D, Meyers J D, Corey L
J Clin Microbiol. 1985 Jul;22(1):95-100. doi: 10.1128/jcm.22.1.95-100.1985.
Sixty-five adenovirus isolates collected over a 3.5-year period were typed by both standard microneutralization techniques and restriction endonuclease digestion of viral DNA. Of the 65 isolates, 47 (72.3%) representing six adenovirus types could be typed by microneutralization. Eighteen isolates demonstrated partial neutralization with standard antisera to two or more adenovirus serotypes and thus could not be definitively typed. DNA analysis permitted typing of 64 of the 65 isolates (98.5%) (including four isolates which contained mixtures of two adenovirus types), and 12 different types were identified. Neutralization and DNA typing disagreed for five isolates, and in each case, digestion with multiple restriction endonucleases and DNA hybridization studies were consistent with the type assigned by DNA analysis. In addition, the DNA analysis method allowed the identification of genomic variants (genome types) of five adenovirus types. We conclude that typing clinical isolates of adenovirus by restriction endonuclease digestion of viral DNA can be done rapidly, provides additional epidemiological and typing information, and provides fewer ambiguous results than does typing by neutralization.
在3.5年的时间里收集的65株腺病毒分离株，通过标准的微量中和技术和病毒DNA的限制性内切酶消化进行分型。在这65株分离株中，47株（72.3%）代表6种腺病毒类型，可通过微量中和进行分型。18株分离株与两种或更多种腺病毒血清型的标准抗血清表现出部分中和反应，因此无法明确分型。DNA分析能够对65株分离株中的64株（98.5%）进行分型（包括4株含有两种腺病毒类型混合物的分离株），并鉴定出12种不同类型。有5株分离株的中和分型与DNA分型不一致，在每种情况下，用多种限制性内切酶消化和DNA杂交研究结果均与DNA分析确定的类型一致。此外，DNA分析方法还能鉴定出5种腺病毒类型的基因组变体（基因组类型）。我们得出结论，通过病毒DNA的限制性内切酶消化对腺病毒临床分离株进行分型能够快速完成，可提供额外的流行病学和分型信息，并且比分型中和法产生的模糊结果更少。
