Shandra A A, Mazarati A M, Servetskii K L
Department of Normal Physiology, N. I. Pirogov Medical Institute, Odessa, Ukraine.
Neurosci Behav Physiol. 1994 Sep-Oct;24(5):429-32. doi: 10.1007/BF02359796.
It was demonstrated in experiments on rats that the injection of the neuropeptide, galanin (200, 500, and 1000 ng), into the lateral cerebral ventricles induced a dose-dependent decrease in the number of successful attempts at avoidance in rats trained preliminarily to active avoidance by jumping. The preliminary administration of the cholinolytic, atropine (1 mg/kg, intraperitoneally), to the rats also caused an acceleration of the damping of the conditioned reflex and potentiated the indicated effect of galanin during the experiment. The use of the opioid antagonist, naloxone (1 mg/kg, intraperitoneally), did not exert an influence on the animals' behavior, but blocked the galanin-provoked acceleration of the extinction of the active avoidance habit. The intraperitoneal administration of a noncompetitive antagonist of excitatory amino acids, ketamine (10 mg/kg), did not influence the character of the animals' behavior nor the indicated effects of galanin. It was concluded that galanin possesses an amnestic action in the active avoidance test, and that this effect of the peptide is determined by the suppression of cholinergic and activation of opiatergic transmission in the central nervous system.
在对大鼠的实验中证明，向侧脑室注射神经肽甘丙肽（200、500和1000纳克）会使预先通过跳跃训练进行主动回避的大鼠的成功回避尝试次数呈剂量依赖性减少。预先给大鼠腹腔注射胆碱能阻滞剂阿托品（1毫克/千克）也会导致条件反射的消退加速，并在实验过程中增强甘丙肽的上述作用。使用阿片样物质拮抗剂纳洛酮（1毫克/千克，腹腔注射）对动物行为没有影响，但会阻断甘丙肽引起的主动回避习惯消退加速。腹腔注射兴奋性氨基酸的非竞争性拮抗剂氯胺酮（10毫克/千克）对动物行为特征以及甘丙肽的上述作用均无影响。得出的结论是，甘丙肽在主动回避试验中具有遗忘作用，且该肽的这种作用是由中枢神经系统中胆碱能传递的抑制和阿片能传递的激活所决定的。
