与猪CD2抗原反应的单克隆抗体分析。
Analysis of monoclonal antibodies reactive with the porcine CD2 antigen.
作者信息
Pescovitz M D, Aasted B, Canals A, Dominguez J, Vizcaino J S, Pospisil R, Sinkora J, Salmon H, Valpotic I, Davis W C
机构信息
Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis 46202.
出版信息
Vet Immunol Immunopathol. 1994 Oct;43(1-3):229-32. doi: 10.1016/0165-2427(94)90140-6.
As result of the First International Swine CD Workshop, six monoclonal antibodies (mAbs) (numbers 014, 023, 024, 057, 128, and 130) clustered closely to the internal standard anti-porcine CD2 mAb, MSA4. Despite the close clustering, the cluster was split into two subgroups. To further characterize the relationship between these mAbs, they were used in flow cytometry to inhibit binding of MSA4 to porcine lymphocytes. mAbs 014 (1038-8-31), 023 (MAC83), 024 (MAC80), 057 (PG168), and 128 (MSA4) completely inhibited the binding of MSA4, mAb 130 (MSA2) failed to inhibit MSA4 binding. On dual parameter flow cytometry comparing MSA2 with MSA4, all MSA2+ cells were MSA4+, two thirds of the MSA4+ cells were MSA2-. We conclude that five of the mAbs bind to the same or a closely related epitope on porcine lymphocytes. mAb 130 appears to have aberrantly clustered with the CD2 group of mAb.
作为第一届国际猪CD研讨会的成果，六种单克隆抗体（mAb）（编号014、023、024、057、128和130）与内标抗猪CD2 mAb MSA4紧密聚类。尽管聚类紧密，但该聚类被分为两个亚组。为了进一步表征这些mAb之间的关系，将它们用于流式细胞术以抑制MSA4与猪淋巴细胞的结合。mAb 014（1038 - 8 - 31）、023（MAC83）、024（MAC80）、057（PG168）和128（MSA4）完全抑制了MSA4的结合，mAb 130（MSA2）未能抑制MSA4的结合。在比较MSA2与MSA4的双参数流式细胞术中，所有MSA2 +细胞均为MSA4 +，三分之二的MSA4 +细胞为MSA2 -。我们得出结论，其中五种mAb与猪淋巴细胞上相同或密切相关的表位结合。mAb 130似乎与CD2组mAb异常聚类。
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