Yoshida T, Ishihara A, Hirokawa Y, Kusakabe M, Sakakura T
Department of Pathology, Mie University School of Medicine, Japan.
Cancer Lett. 1995 Mar 23;90(1):65-73. doi: 10.1016/0304-3835(94)03679-d.
(1) In mouse mammary gland development, immunoreactive tenascin (TN) is expressed in the dense mesenchyme surrounding the epithelial component of 14-day embryos, endbuds at puberty, and tumors. (2) Cells that produce TN are myofibroblastic and are characterized by nuclear invaginations, rough endoplasmic reticulum, and pinocytotic vesicles. These cells are not normally present in the stroma of mammary glands but present in cancer stroma, originating probably from fibroblasts differentiated under the influence of TGF-beta 1 stimulation. (3) Breast cancer cells are capable of synthesing TN under certain conditions. TN-non-producing MCF7 cells can produce TN when co-cultured with embryonic fibroblasts or with their conditioned medium. (4) Nine primary human breast cancers were examined for TN expression by in situ hybridization. TN mRNA was expressed in all nine cases in the stroma and in four cases in carcinoma cells as well. (5) Immunohistochemistry for TN was performed in human breast cancers, and it was found that the five-year survival after surgery was markedly lower in the group whose cancer cells were positive [corrected] for TN. TN expression in cancer cells appears to indicate poor prognosis.
(1) 在小鼠乳腺发育过程中，免疫反应性腱生蛋白（TN）在14天胚胎的上皮成分周围的致密间充质、青春期的终末芽以及肿瘤中表达。(2) 产生TN的细胞是肌成纤维细胞，其特征为核内陷、粗面内质网和吞饮小泡。这些细胞通常不存在于乳腺基质中，但存在于癌基质中，可能起源于在转化生长因子-β1刺激影响下分化的成纤维细胞。(3) 乳腺癌细胞在某些条件下能够合成TN。不产生TN的MCF7细胞与胚胎成纤维细胞或其条件培养基共培养时可产生TN。(4) 通过原位杂交检测了9例原发性人类乳腺癌的TN表达。TN mRNA在所有9例的基质中均有表达，在4例癌细胞中也有表达。(5) 对人类乳腺癌进行了TN免疫组织化学检测，发现癌细胞TN呈阳性[校正后]的组术后五年生存率明显较低。癌细胞中的TN表达似乎预示着预后不良。
