基于16S-23S间隔区的随机扩增多态性DNA分析对结核分枝杆菌菌株进行高效鉴别。
Efficient discrimination of Mycobacterium tuberculosis strains by 16S-23S spacer region-based random amplified polymorphic DNA analysis.
作者信息
Abed Y, Davin-Regli A, Bollet C, De Micco P
机构信息
Hôpital Salvator, Marseille, France.
出版信息
J Clin Microbiol. 1995 May;33(5):1418-20. doi: 10.1128/jcm.33.5.1418-1420.1995.
Abstract
Amplification of the region separating the genes coding for 16S and 23S rRNA was performed with 15 Mycobacterium tuberculosis isolates and the type strain, ATCC 27294. Reproducible amplification patterns were obtained. PCR products were then used as target DNA for random amplified polymorphic DNA (RAPD) analysis. The discriminatory power was higher than when whole genomic DNA was used as a RAPD template. 16S-23S spacer region-based RAPD analysis was a simple and efficient method of differentiation. Consequently, it may be a useful tool for epidemiologic studies of tuberculosis.
摘要
对15株结核分枝杆菌分离株和标准菌株ATCC 27294进行了编码16S和23S rRNA的基因之间区域的扩增。获得了可重复的扩增模式。然后将PCR产物用作随机扩增多态性DNA（RAPD）分析的靶DNA。其鉴别能力高于将全基因组DNA用作RAPD模板时。基于16S - 23S间隔区的RAPD分析是一种简单有效的鉴别方法。因此，它可能是结核病流行病学研究的有用工具。
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