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Can random amplified polymorphic DNA analysis of the 16S-23S spacer region of Mycobacterium tuberculosis differentiate between isolates?对结核分枝杆菌16S - 23S间隔区进行随机扩增多态性DNA分析能否区分不同菌株？
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Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms.基于聚合酶链反应扩增核糖体DNA间隔区多态性的细菌快速鉴定
Appl Environ Microbiol. 1993 Apr;59(4):945-52. doi: 10.1128/aem.59.4.945-952.1993.
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Genomic fingerprinting of "Haemophilus somnus" isolates by using a random-amplified polymorphic DNA assay.利用随机扩增多态性DNA分析对“睡眠嗜血杆菌”分离株进行基因组指纹分析。
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Random amplified polymorphic DNA genotyping of Mycobacterium malmoense.马尔默分枝杆菌的随机扩增多态性DNA基因分型
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Rapid discrimination of Mycobacterium tuberculosis strains by random amplified polymorphic DNA analysis.通过随机扩增多态性DNA分析快速鉴别结核分枝杆菌菌株
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Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.大肠杆菌核糖体RNA操纵子的基因组织与一级结构
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tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.在枯草芽孢杆菌中，转运RNA基因位于16S和23S核糖体RNA基因之间。
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Fingerprinting genomes using PCR with arbitrary primers.使用任意引物通过聚合酶链反应对基因组进行指纹分析。
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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.用任意引物扩增的DNA多态性可作为遗传标记。
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Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis.结核分枝杆菌插入元件IS986：结核病诊断和流行病学的有用工具。
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基于16S-23S间隔区的随机扩增多态性DNA分析对结核分枝杆菌菌株进行高效鉴别。

Efficient discrimination of Mycobacterium tuberculosis strains by 16S-23S spacer region-based random amplified polymorphic DNA analysis.

作者信息

Abed Y, Davin-Regli A, Bollet C, De Micco P

机构信息

Hôpital Salvator, Marseille, France.

出版信息

J Clin Microbiol. 1995 May;33(5):1418-20. doi: 10.1128/jcm.33.5.1418-1420.1995.

DOI:10.1128/jcm.33.5.1418-1420.1995
PMID:7542275
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228183/
Abstract

Amplification of the region separating the genes coding for 16S and 23S rRNA was performed with 15 Mycobacterium tuberculosis isolates and the type strain, ATCC 27294. Reproducible amplification patterns were obtained. PCR products were then used as target DNA for random amplified polymorphic DNA (RAPD) analysis. The discriminatory power was higher than when whole genomic DNA was used as a RAPD template. 16S-23S spacer region-based RAPD analysis was a simple and efficient method of differentiation. Consequently, it may be a useful tool for epidemiologic studies of tuberculosis.

摘要

对15株结核分枝杆菌分离株和标准菌株ATCC 27294进行了编码16S和23S rRNA的基因之间区域的扩增。获得了可重复的扩增模式。然后将PCR产物用作随机扩增多态性DNA（RAPD）分析的靶DNA。其鉴别能力高于将全基因组DNA用作RAPD模板时。基于16S - 23S间隔区的RAPD分析是一种简单有效的鉴别方法。因此，它可能是结核病流行病学研究的有用工具。