Marshall L, Ghosh M M, Boyce S G, MacNeil S, Freedlander E, Kudesia G
Department of Virology, Northern General Hospital, Sheffield, UK.
Burns. 1995 Aug;21(5):356-61. doi: 10.1016/0305-4179(95)00006-2.
Glycerol has long been used for the preservation of skin allografts. The antimicrobial activity of glycerol has not been fully documented. This paper reports the results of an investigation of a model studying the effect of glycerol on the inactivation of intracellular viruses. Two viruses--herpes simplex type I (HSV-1) and poliovirus--were cultured within human dermal fibroblasts. These intracellular viruses were incubated with 50 per cent, 85 per cent and 98 per cent glycerol at 4 degrees C and 20 degrees C for 4 weeks. Each week, the cultures in glycerol and controls in fibroblast maintenance medium were assayed for virus infectivity by examining the ability of harvested viruses to infect further fibroblasts. At 4 degrees C, 85 per cent glycerol could not fully inactivate intracellular HSV-I or poliovirus even after 4 weeks; 98 per cent glycerol inactivated intracellular HSV-I (after 3 weeks) but could not fully inactivate intracellular poliovirus after 4 weeks. At 20 degrees C, 85 per cent glycerol inactivated intracellular HSV-I (within 1 week) but could not fully inactivate intracellular poliovirus after 4 weeks; 98 per cent glycerol inactivated intracellular HSV-I (within 1 week) and inactivated intracellular poliovirus (after 2 weeks). It is suggested that, on the basis of this study, glycerol can reduce intracellular virus infectivity but that its effects are very dependent on concentration, time and temperature such that we would recommend that allograft skin be exposed to 98 per cent glycerol for a minimum of at least 4 weeks at a minimum temperature of 20 degrees C before clinical use.
甘油长期以来一直用于同种异体皮肤移植的保存。甘油的抗菌活性尚未得到充分记录。本文报告了一项模型研究的结果，该研究探讨了甘油对细胞内病毒灭活的影响。两种病毒——单纯疱疹病毒I型（HSV-1）和脊髓灰质炎病毒——在人皮肤成纤维细胞内培养。这些细胞内病毒在4℃和20℃下分别与50%、85%和98%的甘油孵育4周。每周通过检测收获病毒感染更多成纤维细胞的能力，对甘油培养物和在成纤维细胞维持培养基中的对照进行病毒感染性检测。在4℃时，即使经过4周，85%的甘油也不能完全灭活细胞内的HSV-I或脊髓灰质炎病毒；98%的甘油可灭活细胞内的HSV-I（3周后），但4周后不能完全灭活细胞内的脊髓灰质炎病毒。在20℃时，85%的甘油可灭活细胞内的HSV-I（1周内），但4周后不能完全灭活细胞内的脊髓灰质炎病毒；98%的甘油可灭活细胞内的HSV-I（1周内）和细胞内的脊髓灰质炎病毒（2周后）。基于本研究，建议甘油可降低细胞内病毒的感染性，但其效果非常依赖于浓度、时间和温度，因此我们建议在临床使用前，同种异体移植皮肤应在至少20℃的最低温度下，用98%的甘油处理至少4周。
