Richters C D, Hoekstra M J, van Baare J, du Pont J S, Kamperdijk E W
Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
Burns. 1996 Mar;22(2):113-6. doi: 10.1016/0305-4179(95)00103-4.
Donor allograft skin preserved in 85 per cent glycerol has been used successfully as a temporary coverage for large burn wounds. The glycerol preservation is a method with low costs and has practical advantages such as antibacterial and virucidal effects. This report shows that the glycerol treatment did not affect the fundamental structural integrity of the skin. Intact keratinocytes and Langerhans cells with their characteristic Birbeck granules were still present in the glycerol-treated skin. After treatment with glycerol, the cells in the prepared epidermal cell suspensions were non-viable. MHC class II positive and CD1a positive cells could still be identified in situ and in the suspension.
保存在85%甘油中的供体同种异体皮肤已成功用作大面积烧伤创面的临时覆盖物。甘油保存法成本低，具有抗菌和杀病毒等实际优势。本报告表明，甘油处理并未影响皮肤的基本结构完整性。甘油处理过的皮肤中仍存在完整的角质形成细胞和带有特征性伯贝克颗粒的朗格汉斯细胞。甘油处理后，制备的表皮细胞悬液中的细胞无活性。MHC II类阳性细胞和CD1a阳性细胞仍可在原位和悬液中鉴定出来。
