Gueirard P, Weber C, Le Coustumier A, Guiso N
Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France.
J Clin Microbiol. 1995 Aug;33(8):2002-6. doi: 10.1128/jcm.33.8.2002-2006.1995.
Within a period of 2 1/2 years, Bordetella bronchiseptica was isolated four times from a 79-year-old woman with bronchopneumonia. We have demonstrated by pulsed-field gel electrophoresis that this infection was related to contact with infected rabbits. The initial human B. bronchiseptica isolate had a phenotype characteristic of usual B. bronchiseptica clinical isolates; it produced toxin and adhesins, such as adenylate cyclase-hemolysin, filamentous hemagglutinin, and pertactin, and was able to induce lethality in a murine respiratory model. By contrast, although the three successive human isolates produced adhesins, they did not express adenylate cyclase-hemolysin and were unable to induce lethality. This implies that adenylate cyclase-hemolysin is required to induce lethality. We suggest that B. bronchiseptica may persist in the host, with expression of adenylate cyclase-hemolysin being essential for the initiation of infection and expression of adhesins being essential for persistence.
在2年半的时间里，支气管败血波氏杆菌从一名患有支气管肺炎的79岁女性身上被分离出4次。我们通过脉冲场凝胶电泳证明，这种感染与接触受感染的兔子有关。最初从人类分离出的支气管败血波氏杆菌具有普通支气管败血波氏杆菌临床分离株的表型特征；它产生毒素和黏附素，如腺苷酸环化酶溶血素、丝状血凝素和百日咳杆菌黏附素，并且能够在小鼠呼吸道模型中诱导致死性。相比之下，尽管连续的三株人类分离株产生了黏附素，但它们不表达腺苷酸环化酶溶血素，也无法诱导致死性。这意味着腺苷酸环化酶溶血素是诱导致死性所必需的。我们认为支气管败血波氏杆菌可能在宿主体内持续存在，腺苷酸环化酶溶血素的表达对于感染的起始至关重要，而黏附素的表达对于持续存在至关重要。
