Yashiro M, Chung Y S, Nishimura S, Inoue T, Sowa M
First Department of Surgery, Osaka City University Medical School, Japan.
Br J Cancer. 1995 Nov;72(5):1200-10. doi: 10.1038/bjc.1995.486.
Determination of the differences between cell lines which are derived from a primary tumour and a disseminated metastatic lesion from the same patient may aid in elucidating the factors associated with disseminated metastases. We report on the establishment and characterisation of two new scirrhous gastric cancer cell lines, designated OCUM-2M and OCUM-2D, derived from a 49-year-old female. OCUM-2M was derived from a primary gastric tumour, and OCUM-2D was derived from a sample of disseminated metastasis. The two cell lines were derived from the same patient. We investigated biological differences between the two cell lines to study mechanisms involved in disseminated metastasis. The growth activity of OCUM-2D cells as determined by doubling time and tumorigenicity was greater than that of OCUM-2M cells. The level of epidermal growth factor receptor (EGFR) expression in OCUM-2D cells was about twice that of OCUM-2M cells and the growth of OCUM-2D cells was stimulated more by epidermal growth factor (EGF) than that of OCUM-2M cells. The invasive activity of OCUM-2D cells was higher than that of OCUM-2M cells and was increased after addition of transforming growth factor-beta 1 (TGF-beta 1). An increase in the number of attached and spreading cells was found following the addition of 10 ng ml-1 TGF-beta 1. These findings suggest that high growth and invasive activity may play an important role in disseminated metastasis and that EGF and TGF-beta 1, which affect the growth and invasive activity of OCUM-2D cells, might be factors associated with metastasis in scirrhous gastric carcinoma. The two cell lines OCUM-2M and OCUM-2D should be beneficial for analysing mechanisms of tumour progression.
确定源自同一患者原发性肿瘤和播散性转移病灶的细胞系之间的差异，可能有助于阐明与播散性转移相关的因素。我们报告了两个新的硬癌胃癌细胞系OCUM - 2M和OCUM - 2D的建立及特征，它们源自一名49岁女性。OCUM - 2M源自原发性胃肿瘤，OCUM - 2D源自播散性转移样本。这两个细胞系来自同一患者。我们研究了这两个细胞系之间的生物学差异，以探讨参与播散性转移的机制。通过倍增时间和致瘤性测定，OCUM - 2D细胞的生长活性高于OCUM - 2M细胞。OCUM - 2D细胞中表皮生长因子受体（EGFR）的表达水平约为OCUM - 2M细胞的两倍，且表皮生长因子（EGF）对OCUM - 2D细胞生长的刺激作用比对OCUM - 2M细胞更强。OCUM - 2D细胞的侵袭活性高于OCUM - 2M细胞，添加转化生长因子 - β1（TGF - β1）后侵袭活性增加。添加10 ng/ml TGF - β1后，附着和铺展细胞的数量增加。这些发现表明，高生长和侵袭活性可能在播散性转移中起重要作用，影响OCUM - 2D细胞生长和侵袭活性的EGF和TGF - β1可能是硬癌胃癌转移的相关因素。OCUM - 2M和OCUM - 2D这两个细胞系应有助于分析肿瘤进展机制。
