Yoder S, Argueta C, Holtzman A, Aronson T, Berlin O G, Tomasek P, Glover N, Froman S, Stelma G
Education and Research Institute, Olive View-UCLA Medical Center, Sylmar, California 91342, USA.
Appl Environ Microbiol. 1999 Jun;65(6):2650-3. doi: 10.1128/AEM.65.6.2650-2653.1999.
Mycobacterium avium is a cause of disseminated disease in AIDS patients. A need for a better understanding of possible sources and routes of transmission of this organism has arisen. This study utilized a PCR typing method designed to amplify DNA segments located between the insertion sequences IS1245 and IS1311 to compare levels of relatedness of M. avium isolates found in patients and foods. Twenty-five of 121 food samples yielded 29 mycobacterial isolates, of which 12 were M. avium. Twelve food and 103 clinical M. avium isolates were tested. A clinical isolate was found to be identical to a food isolate, and close relationships were found between two patient isolates and two food isolates. Relatedness between food isolates and patient isolates suggests the possibility that food is a potential source of M. avium infection. This study demonstrates a rapid, inexpensive method for typing M. avium, possibly replacing pulsed-field gel electrophoresis.
鸟分枝杆菌是艾滋病患者播散性疾病的一个病因。因此,有必要更好地了解这种病原体可能的来源和传播途径。本研究采用一种聚合酶链反应(PCR)分型方法,该方法旨在扩增插入序列IS1245和IS1311之间的DNA片段,以比较患者和食物中分离出的鸟分枝杆菌菌株的亲缘关系水平。121份食物样本中有25份培养出29株分枝杆菌菌株,其中12株为鸟分枝杆菌。对12份食物来源和103份临床来源的鸟分枝杆菌菌株进行了检测。发现一份临床分离株与一份食物分离株相同,并且在两份患者分离株和两份食物分离株之间发现了密切的亲缘关系。食物分离株与患者分离株之间的亲缘关系表明,食物有可能是鸟分枝杆菌感染的一个潜在来源。本研究展示了一种快速、廉价的鸟分枝杆菌分型方法,可能会取代脉冲场凝胶电泳技术。