新制霉菌素，一种增强多烯类抗真菌抗生素作用的新型抗真菌抗生素。I. 发酵、提取、纯化及理化和生物学性质

Neo-enactin, a new antifungal antibiotic potentiating polyene antifungal antibiotics. I. Fermentation, extraction, purification and physico-chemical and biological properties.

作者信息

Kondo H, Sumomogi H, Otani T, Nakamura S

出版信息

J Antibiot (Tokyo). 1979 Jan;32(1):13-7. doi: 10.7164/antibiotics.32.13.

DOI:10.7164/antibiotics.32.13

PMID:761990

Abstract

A new antifungal antibiotic, named neo-enactin, was isolated from the cultured mycelia of Strain H 829-MY 10, identified as a new subspecies of Streptoverticillium and named Streptoverticillium olivoreticuli subsp. neoenacticus. The antibiotic was produced with a tetraene antifungal antibiotic, found mainly in the cultured mycelia of Strain H 829-MY 10 and it was extracted with methanol. The antibiotic is of a basic nature and it can be extracted with ethyl acetate at alkaline pH. Purification of neo-enactin was carried out by partition chromatography on cellulose and elution with ethyl acetate bufferized with phosphate buffer (pH 8.0). Neo-enactin shows strong antifungal activity and potentiates the antifungal activity of polyene antifungal antibiotics.

摘要

一种名为新恩那汀的新型抗真菌抗生素，是从菌株H 829-MY 10的培养菌丝体中分离出来的，该菌株被鉴定为轮状链霉菌的一个新亚种，并命名为橄榄色轮状链霉菌新恩那汀亚种。该抗生素与一种四烯抗真菌抗生素一起产生，主要存在于菌株H 829-MY 10的培养菌丝体中，并用甲醇提取。该抗生素呈碱性，在碱性pH值下可用乙酸乙酯提取。新恩那汀的纯化是通过在纤维素上进行分配色谱法，并用磷酸盐缓冲液（pH 8.0）缓冲的乙酸乙酯洗脱来进行的。新恩那汀显示出很强的抗真菌活性，并能增强多烯抗真菌抗生素的抗真菌活性。