Tamai S, Kaneda M, Nakamura S
J Antibiot (Tokyo). 1982 Sep;35(9):1130-6. doi: 10.7164/antibiotics.35.1130.
A new antifungal antibiotic, named piperazinomycin, was isolated from the cultured broth of Streptoverticillium olivoreticuli subsp. neoenacticus. The antibiotic was obtained from the mycelial cake by extraction with methanol and also from the broth filtrate by adsorption on Amberlite XAD-2 and subsequent elution with aqueous acetone. The antibitoic is of basic and lipophilic nature and can be extracted with methyl isobutyl ketone at alkaline pH. Its purification was carried out by column chromatography on Sephadex LH-20 and then on Sephadex G-15 followed by preparative thin-layer chromatography on silica gel. The molecular formula of piperazinomycin was determined to be C125H20NsO2 by high resolution mass spectrum and the spectroscopic and chemical properties were examined. Piperazinomycin showed inhibitory activity against fungi and yeasts, especially against Trichophyton.
一种名为哌嗪霉素的新型抗真菌抗生素，是从橄榄网状链霉菌新发酵亚种的培养肉汤中分离出来的。该抗生素可通过用甲醇从菌丝饼中提取获得，也可通过吸附在Amberlite XAD - 2上，随后用丙酮水溶液洗脱从肉汤滤液中获得。该抗生素具有碱性和亲脂性，在碱性pH值下可用甲基异丁基酮提取。其纯化通过在Sephadex LH - 20上进行柱色谱，然后在Sephadex G - 15上进行，随后在硅胶上进行制备性薄层色谱。通过高分辨率质谱测定哌嗪霉素的分子式为C125H20N5O2，并对其光谱和化学性质进行了研究。哌嗪霉素对真菌和酵母显示出抑制活性，尤其是对毛癣菌。
