Kunitake T, Kitamura T, Guo J, Taguchi F, Kawabe K, Yogo Y
Department of Urology, Fuchu Metropolitan Hospital, Tokyo, Japan.
J Clin Microbiol. 1995 Jun;33(6):1448-51. doi: 10.1128/jcm.33.6.1448-1451.1995.
JC polyomavirus (JCV), the causative agent of progressive multifocal leukoencephalopathy, is ubiquitous in the human population, infecting children asymptomatically and then persisting in renal tissue. In most adults, renal JCV replicates and the progeny are excreted in urine. We used this urinary JCV to elucidate the routes of JCV transmission. A 610-bp JCV DNA region (IG region) encompassing the 3'-terminal sequences of both T-antigen and VP1 (major capsid protein) genes was amplified by means of PCR from urine specimens collected from all members of seven families. JCV strains were then unequivocally identified by the nucleotide sequences of the amplified IG regions. We could identify 18 distinctive JCV strains from 27 individuals. Different JCV strains were detected from all unrelated persons. However, the same viral strain was detected from one (four families), two (one family), or three offspring (one family) as well as from the fathers (three families) or from the mothers (three families). In total, the JCV strains detected in half of the JCV-positive children were identified in their parents. Since most humans are infected during childhood, these findings indicated that JCV is transmitted frequently from parents to children. We roughly estimated that 50% of JCV transmission occurs by this route and that the other 50% occurs outside the family.
JC多瘤病毒（JCV）是进行性多灶性白质脑病的病原体，在人群中普遍存在，儿童感染后无症状，随后病毒持续存在于肾组织中。在大多数成年人中，肾内的JCV会复制，子代病毒会随尿液排出。我们利用这种尿液中的JCV来阐明JCV的传播途径。通过PCR从七个家庭所有成员收集的尿液标本中扩增出一个610bp的JCV DNA区域（IG区域），该区域包含T抗原和VP1（主要衣壳蛋白）基因的3'末端序列。然后通过扩增的IG区域的核苷酸序列明确鉴定JCV毒株。我们从27个人中鉴定出18种不同的JCV毒株。所有无关个体均检测到不同的JCV毒株。然而，在一个家庭的一个孩子（四个家庭）、两个孩子（一个家庭）或三个孩子（一个家庭）以及父亲（三个家庭）或母亲（三个家庭）中检测到相同的病毒株。总共，在一半JCV阳性儿童中检测到的JCV毒株在其父母中也被鉴定出来。由于大多数人在儿童时期就受到感染，这些发现表明JCV经常从父母传播给孩子。我们粗略估计，50%的JCV传播是通过这种途径发生的，另外50%发生在家庭之外。
