Berthelot P, Grattard F, Mahul P, Jospe R, Pozzetto B, Ros A, Gaudin O G, Auboyer C
Department of Microbiology, Faculty of Medicine J. Lisfranc, University of Saint-Etienne, France.
J Hosp Infect. 1993 Sep;25(1):33-43. doi: 10.1016/0195-6701(93)90006-l.
A prospective study was undertaken to determine the source of Pseudomonas cepacia colonization and infection that had affected ventilated patients in an Intensive Care Unit (ICU) for three years. Thirty-eight patients undergoing mechanical ventilation were enrolled during a six-week period. Samples were taken from patients, ventilator circuits and the environment for culture. P. cepacia was isolated from the condensate formed in the ventilator circuit and the source of the contamination was shown to be the temperature sensor. Ribotyping of the representative strains of P. cepacia performed with two endonucleases, EcoRI and PvuII, confirmed the homogeneity of the isolates from patients and ventilator circuits. A modification of the procedure for disinfection of the temperature sensors resulted in the eradication of P. cepacia from the ICU.
开展了一项前瞻性研究，以确定在重症监护病房（ICU）影响接受机械通气患者长达三年的洋葱伯克霍尔德菌定植和感染的来源。在六周内招募了38名接受机械通气的患者。从患者、呼吸机回路和环境中采集样本进行培养。在呼吸机回路形成的冷凝物中分离出洋葱伯克霍尔德菌，且污染来源为温度传感器。用两种核酸内切酶EcoRI和PvuII对洋葱伯克霍尔德菌的代表性菌株进行核糖分型，证实了来自患者和呼吸机回路的分离株具有同质性。对温度传感器消毒程序的改进导致ICU中洋葱伯克霍尔德菌的根除。
