Nøorgaard A, Andersen L P, Nielsen H
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Gut. 1995 Mar;36(3):354-7. doi: 10.1136/gut.36.3.354.
Mucosal biopsy specimens from patients with Helicobacter pylori infection in gastric antrum contain an increased amount of myeloperoxidase. This study was performed to elucidate the interaction of H pylori sonicate protein(s) and neutrophils concerning myeloperoxidase release. Neutrophil degranulation with myeloperoxidase release was examined in a direct stimulating assay. Priming activity of H pylori was examined after preincubating neutrophils in sonicate, either crude or modified by heat treatment, pronase inactivation and dialysis, and stimulating with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or serum opzonised zymosan (OZ). It was found that H pylori sonicate protein(s) stimulates neutrophil degranulation with myeloperoxidase release in a concentration dependent way. The activity was distinct from fMLP and capable of priming the subsequent fMLP and OZ response. Experiments with the modified bacterial sonicate suggest the activity is caused by a protein, but the findings show that non-protein molecules, for example, lipopolysaccarides were also part of the H pylori sonicate priming activity. The increased mucosal myeloperoxidase in H pylori associated disease can be a direct consequence of bacteria derived stimulation of inflammatory neutrophils.
胃窦幽门螺杆菌感染患者的黏膜活检标本中髓过氧化物酶含量增加。本研究旨在阐明幽门螺杆菌超声裂解蛋白与中性粒细胞在髓过氧化物酶释放方面的相互作用。在直接刺激试验中检测了中性粒细胞脱颗粒及髓过氧化物酶释放情况。在将中性粒细胞与未经处理的粗制超声裂解物、经热处理、链霉蛋白酶失活和透析处理的超声裂解物预孵育后，用N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）或血清调理酵母聚糖（OZ）刺激，检测幽门螺杆菌的启动活性。结果发现，幽门螺杆菌超声裂解蛋白以浓度依赖的方式刺激中性粒细胞脱颗粒并释放髓过氧化物酶。该活性不同于fMLP，且能够启动随后的fMLP和OZ反应。对经处理的细菌超声裂解物进行的实验表明，该活性由一种蛋白质引起，但研究结果表明，非蛋白质分子，如脂多糖，也是幽门螺杆菌超声裂解物启动活性的一部分。幽门螺杆菌相关疾病中黏膜髓过氧化物酶增加可能是细菌刺激炎性中性粒细胞的直接结果。
