处理样本量对通过聚合酶链反应检测泌尿生殖系统样本中沙眼衣原体的影响。
Influence of volume of sample processed on detection of Chlamydia trachomatis in urogenital samples by PCR.
作者信息
Goessens W H, Kluytmans J A, den Toom N, van Rijsoort-Vos T H, Niesters B G, Stolz E, Verbrugh H A, Quint W G
机构信息
Department of Clinical Microbiology, Erasmus University, Rotterdam, The Netherlands.
出版信息
J Clin Microbiol. 1995 Jan;33(1):251-3. doi: 10.1128/jcm.33.1.251-253.1995.
Abstract
In the present study, it was demonstrated that the sensitivity of the PCR for the detection of Chlamydia trachomatis is influenced by the volume of the clinical sample which is processed in the PCR. An adequate sensitivity for PCR was established by processing at least 4%, i.e., 80 microliters, of the clinical sample volume per PCR. By using this preparation procedure, 1,110 clinical samples were evaluated by PCR and by cell culture, and results were compared. After discordant analysis, cell culture resulted in a sensitivity of 79.1% and PCR resulted in a sensitivity of 92.7%. Furthermore, it was shown that treatment with antibiotics immediately resulted in negative cell culture results but that PCR could give positive results up to 2 weeks posttreatment.
摘要
在本研究中，已证明用于检测沙眼衣原体的聚合酶链反应（PCR）的灵敏度受PCR中所处理临床样本体积的影响。通过每次PCR处理至少4%，即80微升的临床样本体积，可建立PCR的足够灵敏度。采用此制备程序，通过PCR和细胞培养对1110份临床样本进行了评估，并对结果进行了比较。经过不一致性分析，细胞培养的灵敏度为79.1%，PCR的灵敏度为92.7%。此外，研究表明，抗生素治疗立即导致细胞培养结果为阴性，但PCR在治疗后2周内仍可能给出阳性结果。
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