运用沙眼衣原体扩增检测法检测男性和女性尿液样本中的沙眼衣原体。
Detection of Chlamydia trachomatis in male and female urine specimens by using the amplified Chlamydia trachomatis test.
作者信息
Mouton J W, Verkooyen R, van der Meijden W I, van Rijsoort-Vos T H, Goessens W H, Kluytmans J A, Deelen S D, Luijendijk A, Verbrugh H A
机构信息
Department of Medical Microbiology and Infectious Diseases, Erasmus University Hospital Rotterdam, The Netherlands.
出版信息
J Clin Microbiol. 1997 Jun;35(6):1369-72. doi: 10.1128/jcm.35.6.1369-1372.1997.
The amplified Chlamydia trachomatis test (AMP-CT; Gen-Probe), a new diagnostic test for the detection of Chlamydia trachomatis, was evaluated with urine specimens from 1,000 patients visiting the outpatient department for sexually transmitted diseases at the University Hospital Rotterdam, Rotterdam, The Netherlands, by comparing the results to those of cell culture. From February 1996 to July 1996, urine samples for the AMP-CT test and urethral swabs for cell culture were collected from 544 men, while cervical swabs from 456 women were also taken for cell culture. Positive test results were obtained for 130 (13%) of the patients. AMP-CT test and cell culture results were discordant for 70 (7%) specimens. Analysis of the samples with discordant results was performed by an in-house PCR. After resolution of the discordant results, the sensitivity, specificity, and positive and negative predictive values of the AMP-CT test were 84.3, 98.8, 89.6, and 98%, respectively, for samples from females and 100, 99.2, 93.1, and 100%, respectively, for samples from males, while for cell culture these values were 72.5, 99.2, 92.5, and 98%, respectively, for samples from females and 57.4, 99.0, 86.1, and 95.4%, respectively, for samples from males. We conclude that the AMP-CT test is a fast and reliable test for the detection of C. trachomatis in urine specimens from females and, in particular, males.
扩增沙眼衣原体检测（AMP-CT；基因探针公司）是一种用于检测沙眼衣原体的新型诊断测试。我们在荷兰鹿特丹大学医院对1000名前来门诊就诊的性传播疾病患者的尿液样本进行了评估，将检测结果与细胞培养结果进行比较。1996年2月至1996年7月，收集了544名男性的尿液样本用于AMP-CT检测，并采集尿道拭子用于细胞培养，同时还采集了456名女性的宫颈拭子用于细胞培养。130名（13%）患者检测结果呈阳性。70份（7%）样本的AMP-CT检测结果与细胞培养结果不一致。对结果不一致的样本采用内部聚合酶链反应（PCR）进行分析。在解决结果不一致的问题后，AMP-CT检测对于女性样本的敏感性、特异性、阳性预测值和阴性预测值分别为84.3%、98.8%、89.6%和98%，对于男性样本分别为100%、99.2%、93.1%和100%；而细胞培养对于女性样本的这些值分别为72.5%、99.2%、92.5%和98%，对于男性样本分别为57.4%、99.0%、86.1%和95.4%。我们得出结论，AMP-CT检测是一种快速且可靠的检测方法，可用于检测女性尤其是男性尿液样本中的沙眼衣原体。
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