Armstrong K A, Fan D P
J Bacteriol. 1976 Apr;126(1):48-55. doi: 10.1128/jb.126.1.48-55.1976.
We isolated 25 independent mutants that are deficient in essential genes located in the metB-malB region of the Escherichia coli chromosome. The mutations were mapped within this region by using several F' factors and were also classified into 11 cistrons by complementation testing. There is clustering of mutations in essential genes within the metB-malB region.
我们分离出了25个独立的突变体,它们在大肠杆菌染色体metB-malB区域的必需基因中存在缺陷。利用几个F'因子将这些突变定位在该区域内,并且通过互补测试也将它们分为11个顺反子。metB-malB区域内必需基因的突变存在聚类现象。