Vallyathan V
Pathology Section, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505.
Environ Health Perspect. 1994 Dec;102 Suppl 10(Suppl 10):111-5. doi: 10.1289/ehp.94102s10111.
Occupational exposure to mineral dust causes pneumoconiosis and other diseases. A cytotoxicity assay to predict the potential of minerals to cause disease would be of great value as a prevention strategy. This study compares the ability of several minerals to generate the more potent oxidizing agent, hydroxyl radical (.OH), and their cytotoxicity and lipid peroxidation potentials. Crystalline silica, the most potent cytotoxic and pathogenic mineral studied, showed the least ability to generate .OH radicals while inducing the maximal lipid peroxidation. Coal mine dust, showing the maximal ability to generate .OH radicals, was the least cytotoxic in bioassays of toxicity and induction of lipid peroxidation. Based on these results, it would appear that the ability of minerals to induce lipid peroxidation provides a better correlation with known cytotoxicity and pathogenicity of minerals than does their ability to generate oxygen radicals.
职业接触矿物粉尘会导致尘肺病和其他疾病。作为一种预防策略，一种用于预测矿物致病潜力的细胞毒性测定方法将具有重要价值。本研究比较了几种矿物产生更强氧化剂羟基自由基（·OH）的能力，以及它们的细胞毒性和脂质过氧化潜力。结晶二氧化硅是所研究的最具细胞毒性和致病性的矿物，在诱导最大脂质过氧化的同时，产生·OH自由基的能力最弱。煤矿粉尘产生·OH自由基的能力最强，在毒性生物测定和脂质过氧化诱导方面细胞毒性最小。基于这些结果，似乎矿物诱导脂质过氧化的能力与其已知的细胞毒性和致病性之间的相关性，比其产生氧自由基的能力更好。
