含有焦油自由基的香烟焦油的水提取物会导致哺乳动物细胞中的DNA切口。
Aqueous extracts of cigarette tar containing the tar free radical cause DNA nicks in mammalian cells.
作者信息
Stone K K, Bermúdez E, Pryor W A
机构信息
Biodynamics Institute, Louisiana State University, Baton Rouge 70803-1800.
出版信息
Environ Health Perspect. 1994 Dec;102 Suppl 10(Suppl 10):173-8. doi: 10.1289/ehp.94102s10173.
Abstract
The ability of aqueous extracts of cigarette tar to nick DNA was investigated using viable mammalian cells. Tar extracts contain a radical with a stable electron spin resonance (ESR) signal at g = 2.0036 characteristic of a semiquinone. The association of the tar component that carries the ESR signal with DNA was demonstrated using viable rat alveolar macrophages. The formation of single-strand DNA breaks caused by cigarette tar extracts in viable rat thymocytes follows saturation kinetics, indicating a tar component associates with DNA and then nicks it. These studies support our hypothesis that tar components that contain the cigarette tar radical can enter cells, associate with, and then nick DNA.
摘要
使用活的哺乳动物细胞研究了香烟焦油的水提取物切割DNA的能力。焦油提取物含有一种自由基，其在g = 2.0036处具有稳定的电子自旋共振（ESR）信号，这是半醌的特征。使用活的大鼠肺泡巨噬细胞证明了携带ESR信号的焦油成分与DNA的结合。香烟焦油提取物在活的大鼠胸腺细胞中引起的单链DNA断裂的形成遵循饱和动力学，表明焦油成分与DNA结合然后切割它。这些研究支持了我们的假设，即含有香烟焦油自由基的焦油成分可以进入细胞，与DNA结合，然后切割DNA。
相似文献
1
Aqueous extracts of cigarette tar containing the tar free radical cause DNA nicks in mammalian cells.含有焦油自由基的香烟焦油的水提取物会导致哺乳动物细胞中的DNA切口。
Environ Health Perspect. 1994 Dec;102 Suppl 10(Suppl 10):173-8. doi: 10.1289/ehp.94102s10173.
2
The ESR properties, DNA nicking, and DNA association of aged solutions of catechol versus aqueous extracts of tar from cigarette smoke.儿茶酚老化溶液与香烟烟雾焦油的水提取物的电子自旋共振特性、DNA切口及DNA结合情况。
Arch Biochem Biophys. 1995 May 10;319(1):196-203. doi: 10.1006/abbi.1995.1282.
3
Fractionation of aqueous cigarette tar extracts: fractions that contain the tar radical cause DNA damage.香烟焦油提取物的分级分离：含有焦油自由基的级分可导致DNA损伤。
Chem Res Toxicol. 1998 May;11(5):441-8. doi: 10.1021/tx970159y.
4
Environmental tobacco smoke is just as damaging to DNA as mainstream smoke.
Environ Health Perspect. 1994 Oct;102(10):870-4. doi: 10.1289/ehp.102-1567343.
5
Oxygen consumption and oxyradical production from microsomal reduction of aqueous extracts of cigarette tar.香烟焦油水提取物微粒体还原过程中的耗氧量和氧自由基生成量。
Arch Biochem Biophys. 1993 Aug 1;304(2):371-8. doi: 10.1006/abbi.1993.1364.
6
A comparative study by electron paramagnetic resonance of free radical species in the mainstream and sidestream smoke of cigarettes with conventional acetate filters and 'bio-filters'.一项通过电子顺磁共振对带有传统醋酸纤维滤嘴和“生物滤嘴”香烟主流烟气和侧流烟气中自由基种类进行的对比研究。
Redox Rep. 2001;6(3):161-71. doi: 10.1179/135100001101536274.
7
Cigarette tar causes single-strand breaks in DNA.
Biochem Biophys Res Commun. 1985 Dec 17;133(2):780-6. doi: 10.1016/0006-291x(85)90972-6.
8
Cigarette smoke radicals and the role of free radicals in chemical carcinogenicity.香烟烟雾自由基以及自由基在化学致癌作用中的角色。
Environ Health Perspect. 1997 Jun;105 Suppl 4(Suppl 4):875-82. doi: 10.1289/ehp.97105s4875.
9
DNA synthesis is blocked by cigarette tar-induced DNA single-strand breaks.
Carcinogenesis. 1987 Oct;8(10):1517-20. doi: 10.1093/carcin/8.10.1517.
10
Biological effects of cigarette smoke, wood smoke, and the smoke from plastics: the use of electron spin resonance.香烟烟雾、木材烟雾和塑料烟雾的生物学效应：电子自旋共振的应用
Free Radic Biol Med. 1992 Dec;13(6):659-76. doi: 10.1016/0891-5849(92)90040-n.
引用本文的文献
1
Signaling pathways of oxidative stress response: the potential therapeutic targets in gastric cancer.氧化应激反应信号通路：胃癌治疗的潜在靶点。
Front Immunol. 2023 Apr 18;14:1139589. doi: 10.3389/fimmu.2023.1139589. eCollection 2023.
2
Relationships among smoking, oxidative stress, inflammation, macromolecular damage, and cancer.吸烟、氧化应激、炎症、大分子损伤与癌症之间的关系。
Mutat Res Rev Mutat Res. 2021 Jan-Jun;787:108365. doi: 10.1016/j.mrrev.2021.108365. Epub 2021 Jan 11.
3
Lung Macrophage Functional Properties in Chronic Obstructive Pulmonary Disease.慢性阻塞性肺疾病中的肺巨噬细胞功能特性。
Int J Mol Sci. 2020 Jan 28;21(3):853. doi: 10.3390/ijms21030853.
4
Smoking and Male Infertility: An Evidence-Based Review.吸烟与男性不育：一项基于证据的综述。
World J Mens Health. 2015 Dec;33(3):143-60. doi: 10.5534/wjmh.2015.33.3.143. Epub 2015 Dec 23.
5
Cigarette smoke causes caspase-independent apoptosis of bronchial epithelial cells from asthmatic donors.香烟烟雾可导致哮喘患者供体的支气管上皮细胞发生非半胱天冬酶依赖性凋亡。
PLoS One. 2015 Mar 20;10(3):e0120510. doi: 10.1371/journal.pone.0120510. eCollection 2015.
6
The implication of cigarette smoking and cessation on macrophage cholesterol efflux in coronary artery disease patients.吸烟与戒烟对冠心病患者巨噬细胞胆固醇流出的影响。
J Lipid Res. 2015 Mar;56(3):682-691. doi: 10.1194/jlr.P055491. Epub 2015 Jan 19.
7
Environmental health hazards of e-cigarettes and their components: Oxidants and copper in e-cigarette aerosols.电子烟及其成分对环境健康的危害：电子烟气溶胶中的氧化剂和铜
Environ Pollut. 2015 Mar;198:100-7. doi: 10.1016/j.envpol.2014.12.033. Epub 2015 Jan 9.
8
The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice.香烟烟雾提取物对小鼠排卵、卵母细胞形态及卵巢基因表达的影响。
PLoS One. 2014 Apr 28;9(4):e95945. doi: 10.1371/journal.pone.0095945. eCollection 2014.
9
Effects of (-)-epigallocatechin gallate (EGCG) on DNA strand breaks as evaluated by single-cell gel electrophoresis (SCG) in human lymphocytes.(-)-表没食子儿茶素没食子酸酯 (EGCG) 通过单细胞凝胶电泳 (SCG) 评价对人淋巴细胞 DNA 链断裂的影响。
Environ Health Prev Med. 2001 Jan;5(4):150-4. doi: 10.1007/BF02918291.
10
Coordinate control of expression of Nrf2-modulated genes in the human small airway epithelium is highly responsive to cigarette smoking.人小气道上皮中Nrf2调节基因表达的协同控制对吸烟高度敏感。
Mol Med. 2009 Jul-Aug;15(7-8):203-19. doi: 10.2119/molmed.2008.00130. Epub 2009 Mar 20.
本文引用的文献
1
Oxidants in cigarette smoke. Radicals, hydrogen peroxide, peroxynitrate, and peroxynitrite.香烟烟雾中的氧化剂。自由基、过氧化氢、过氧硝酸盐和过氧亚硝酸盐。
Ann N Y Acad Sci. 1993 May 28;686:12-27; discussion 27-8. doi: 10.1111/j.1749-6632.1993.tb39148.x.
2
Extracellular release of hydrogen peroxide by human alveolar macrophages: the relationship to cigarette smoking and lower respiratory tract infections.人肺泡巨噬细胞过氧化氢的细胞外释放：与吸烟及下呼吸道感染的关系
Clin Sci (Lond). 1983 Dec;65(6):661-4. doi: 10.1042/cs0650661.
3
The radicals in cigarette tar: their nature and suggested physiological implications.香烟焦油中的自由基：其性质及潜在的生理影响。
Science. 1983 Apr 22;220(4595):425-7. doi: 10.1126/science.6301009.
4
Electron-spin resonance study of mainstream and sidestream cigarette smoke: nature of the free radicals in gas-phase smoke and in cigarette tar.主流和侧流香烟烟雾的电子自旋共振研究：气相烟雾和香烟焦油中自由基的性质
Environ Health Perspect. 1983 Jan;47:345-55. doi: 10.1289/ehp.8347345.
5
Altered oxidative metabolic responses in vitro of alveolar macrophages from asymptomatic cigarette smokers.无症状吸烟者肺泡巨噬细胞体外氧化代谢反应的改变。
Am Rev Respir Dis. 1981 Jan;123(1):85-9. doi: 10.1164/arrd.1981.123.1.85.
6
Enhanced cytotoxic potential of alveolar macrophages from cigarette smokers.
J Lab Clin Med. 1988 Mar;111(3):293-8.
7
Cigarette smoke and the involvement of free radical reactions in chemical carcinogenesis.香烟烟雾与自由基反应在化学致癌作用中的参与
Br J Cancer Suppl. 1987 Jun;8:19-23.
8
Free-radical chemistry of cigarette smoke and its toxicological implications.香烟烟雾的自由基化学及其毒理学意义。
Environ Health Perspect. 1985 Dec;64:111-26. doi: 10.1289/ehp.8564111.
9
Cigarette tar causes single-strand breaks in DNA.
Biochem Biophys Res Commun. 1985 Dec 17;133(2):780-6. doi: 10.1016/0006-291x(85)90972-6.
10
Pulmonary macrophages: alveolar and interstitial populations.肺巨噬细胞：肺泡和间质群体。
Exp Lung Res. 1985;9(3-4):177-90. doi: 10.3109/01902148509057522.
Zotero 插件