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大肠杆菌dnaZ基因产物参与体外DNA延伸过程。

Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro.

作者信息

Wickner S, Hurwitz J

出版信息

Proc Natl Acad Sci U S A. 1976 Apr;73(4):1053-7. doi: 10.1073/pnas.73.4.1053.

DOI:10.1073/pnas.73.4.1053
PMID:772677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430198/
Abstract

E. coli dnaZ gene product is required for conversion of phiX174, fd, and ST-1 single-stranded phage DNAs to duplex DNAs in vitro. This protein has been purified about 5000-fold. It functions in the elongation of RNA- or DNA-primed single-stranded DNA that is catalyzed by DNA polymerase III(DNA nucleotidyltransferase; deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase; EC 2.7.7.7) in conjunctions with two other E. coli protein preparations referred to as DNA elongation factors I and III. It also functions in similar reactions catalyzed by DNA polymerase II in combination with E. coli DNA binding protein and DNA elongation factors I and III.

摘要

大肠杆菌dnaZ基因产物是体外将φX174、fd和ST - 1单链噬菌体DNA转化为双链DNA所必需的。这种蛋白质已被纯化了约5000倍。它在由DNA聚合酶III（DNA核苷酸转移酶；脱氧核苷三磷酸：DNA脱氧核苷酸转移酶；EC 2.7.7.7）催化的RNA或DNA引发的单链DNA延伸过程中发挥作用，与另外两种被称为DNA延伸因子I和III的大肠杆菌蛋白质制剂协同作用。它也在由DNA聚合酶II与大肠杆菌DNA结合蛋白以及DNA延伸因子I和III共同催化的类似反应中发挥作用。

相似文献

1
Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro.大肠杆菌dnaZ基因产物参与体外DNA延伸过程。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1053-7. doi: 10.1073/pnas.73.4.1053.
2
Mechanism of DNA elongation catalyzed by Escherichia coli DNA polymerase III, dnaZ protein, and DNA elongation factors I and III.大肠杆菌DNA聚合酶III、dnaZ蛋白以及DNA延伸因子I和III催化的DNA延伸机制。
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3511-5. doi: 10.1073/pnas.73.10.3511.
3
Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.用纯化的大肠杆菌蛋白将φX174病毒DNA转化为双链形式。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4. doi: 10.1073/pnas.71.10.4120.
4
The dnaZ protein, the gamma subunit of DNA polymerase III holoenzyme of Escherichia coli.dnaZ蛋白，即大肠杆菌DNA聚合酶III全酶的γ亚基。
J Biol Chem. 1980 Dec 25;255(24):11698-703.
5
Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.两种蛋白质因子和ATP参与大肠杆菌DNA聚合酶3催化的体外DNA合成。
Proc Natl Acad Sci U S A. 1974 Jan;71(1):6-10. doi: 10.1073/pnas.71.1.6.
6
dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.dnaG基因产物，一种耐利福平的RNA聚合酶，启动单链大肠杆菌噬菌体DNA向其双链复制形式的转化。
J Biol Chem. 1975 Aug 10;250(15):5995-6001.
7
Initiation of DNA replication by the dnaG protein.由dnaG蛋白引发的DNA复制
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The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.大肠杆菌的脱氧核糖核酸解旋蛋白。复制过程中的性质与功能。
J Biol Chem. 1975 Mar 25;250(6):1972-80.
9
Selective inhibition of in vitro DNA synthesis dependent on phiX174 compared with fd DNA. I. Protein requirements for selective inhibition.
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10
A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.从大肠杆菌中分离出的一种DNA解旋蛋白：它与DNA及DNA聚合酶的相互作用。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3537-41. doi: 10.1073/pnas.69.12.3537.

引用本文的文献

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Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8376-80. doi: 10.1073/pnas.121009498.
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The Escherichia coli DNA polymerase III holoenzyme contains both products of the dnaX gene, tau and gamma, but only tau is essential.大肠杆菌DNA聚合酶III全酶包含dnaX基因的两种产物，即τ和γ，但只有τ是必需的。
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Mutations in Escherichia coli dnaA which suppress a dnaX(Ts) polymerization mutation and are dominant when located in the chromosomal allele and recessive on plasmids.大肠杆菌dnaA中的突变，可抑制dnaX（Ts）聚合突变，位于染色体等位基因时呈显性，而在质粒上则呈隐性。
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Conditional lethality of Escherichia coli strains carrying dnaE and dnaQ mutations.携带dnaE和dnaQ突变的大肠杆菌菌株的条件致死性。
Mol Gen Genet. 1981;181(1):24-8. doi: 10.1007/BF00339000.
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Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
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6
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Cloning of the Escherichia coli dnaZX region and identification of its products.大肠杆菌dnaZX区域的克隆及其产物的鉴定。
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Overproduction of Escherichia coli replication proteins by the use of runaway-replication plasmids.通过使用失控复制质粒过量生产大肠杆菌复制蛋白。
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DNA polymerases in prokaryotes and eukaryotes: mode of action and biological implications.原核生物和真核生物中的DNA聚合酶：作用模式及生物学意义
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10
Interaction of the Escherichia coli dnaA initiation protein with the dnaZ polymerization protein in vivo.大肠杆菌dnaA起始蛋白与dnaZ聚合蛋白在体内的相互作用。
Proc Natl Acad Sci U S A. 1982 May;79(10):3340-4. doi: 10.1073/pnas.79.10.3340.

本文引用的文献

1
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
2
Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.脱氧核糖核酸的酶促合成。二十六。一种纯一的脱氧核糖核酸聚合酶的物理和化学研究。
J Biol Chem. 1969 Jun 10;244(11):2996-3008.
3
Multienzyme systems of DNA replication.DNA复制的多酶系统
Science. 1974 Dec 13;186(4168):987-93. doi: 10.1126/science.186.4168.987.
4
Growth of phages lambda, phiX174, and Ml3 requires the dnaZ (previously dnaH) gene product of Escherichia coli.噬菌体λ、φX174和M13的生长需要大肠杆菌的dnaZ（以前称为dnaH）基因产物。
Biochem Biophys Res Commun. 1974 Dec 11;61(3):1036-42. doi: 10.1016/0006-291x(74)90259-9.
5
Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.用纯化的大肠杆菌蛋白将φX174病毒DNA转化为双链形式。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4. doi: 10.1073/pnas.71.10.4120.
6
A holoenzyme form of deoxyribonucleic acid polymerase III. Isolation and properties.脱氧核糖核酸聚合酶III的全酶形式。分离及性质
J Biol Chem. 1974 Oct 10;249(19):6244-9.
7
Conversion of the M13 viral single strand to the double-stranded replicative forms by purified proteins.通过纯化蛋白将M13病毒单链转化为双链复制形式。
J Biol Chem. 1974 Jul 10;249(13):3999-4005.
8
Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli.细菌细胞分裂调控：大肠杆菌dnaH基因座的特性分析
J Bacteriol. 1974 Aug;119(2):443-9. doi: 10.1128/jb.119.2.443-449.1974.
9
Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.两种蛋白质因子和ATP参与大肠杆菌DNA聚合酶3催化的体外DNA合成。
Proc Natl Acad Sci U S A. 1974 Jan;71(1):6-10. doi: 10.1073/pnas.71.1.6.
10
A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template.一种新型DNA聚合酶3和一种共聚合酶复制一条长的单链引物模板。
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1764-7. doi: 10.1073/pnas.70.6.1764.