Püschel A W, Adams R H, Betz H
Abteilung Neurochemie Max-Planck-Institut für Hirnforschung, Frankfurt am Main, Federal Republic of Germany.
Neuron. 1995 May;14(5):941-8. doi: 10.1016/0896-6273(95)90332-1.
Members of the collapsin/semaphorin gene family have been proposed to act as growth cone guidance signals in vertebrates and invertebrates. To identify candidate molecules involved in axonal pathfinding during mouse embryogenesis, we isolated cDNAs encoding five new members of the semaphorin family (Sem A-Sem E). The murine semaphorin genes are differentially expressed in mesoderm and neuroectoderm before and during the time when axons select their pathways in the embryo. In explant cultures, recombinant Sem D/collapsin converts a matrix permissive for axonal growth into one that is inhibitory for neurites of peripheral ganglia. Our data demonstrate that semaphorins are a diverse family of molecules that may provide local signals to specify territories nonaccessible for growing axons.
有人提出，塌陷蛋白/信号素基因家族的成员在脊椎动物和无脊椎动物中充当生长锥导向信号。为了鉴定在小鼠胚胎发育过程中参与轴突寻路的候选分子，我们分离出了编码信号素家族五个新成员（信号素A - 信号素E）的cDNA。在胚胎中轴突选择其路径之前和期间，小鼠信号素基因在中胚层和神经外胚层中差异表达。在植块培养中，重组信号素D/塌陷蛋白将允许轴突生长的基质转变为对外周神经节神经突具有抑制作用的基质。我们的数据表明，信号素是一个多样的分子家族，可能提供局部信号以指定生长中的轴突无法到达的区域。
