来自第二条染色体右臂的12.5千碱基序列预测了IRA1蛋白的一个新的N端序列，并揭示了两个新基因，其中一个是DEAD-box解旋酶。

The sequence of 12.5 kb from the right arm of chromosome II predicts a new N-terminal sequence for the IRA1 protein and reveals two new genes, one of which is a DEAD-box helicase.

作者信息

Zagulski M, Bécam A M, Grzybowska E, Lacroute F, Migdalski A, Slonimski P P, Sokolowska B, Herbert C J

机构信息

Centre de Génétique Moléculaire, Laboratoire propre du CNRS associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.

出版信息

Yeast. 1994 Sep;10(9):1227-34. doi: 10.1002/yea.320100911.

DOI:10.1002/yea.320100911

PMID:7754712

Abstract

We have determined the complete nucleotide sequence of a 12.5 kb segment from the right arm of chromosome II carried by the cosmid alpha 20. The sequence encodes the 5' end of the IRA1 gene. Two complete new open reading frames and the 3' non-coding region of the SUP1 (SUP45) gene. A comparison of our sequence with the data bank reveals a 154 amino acid extension at the N-terminus of Ira1p compared to the previously predicted sequence. According to the 11th edition of the Saccharomyces cerevisiae genetic map, our sequence should encode the MAK5 gene, which is necessary for the maintenance of dsRNA killer plasmids. One of the two new open reading frames, YBR1119, is predicted to encode an RNA helicase, thus YBR1119 may correspond to the MAK5 gene.

摘要

我们已经确定了黏粒α20所携带的来自II号染色体右臂的一个12.5 kb片段的完整核苷酸序列。该序列编码IRA1基因的5'端、两个全新的开放阅读框以及SUP1（SUP45）基因的3'非编码区。将我们的序列与数据库数据进行比较后发现，与之前预测的序列相比，Ira1p的N端有一个154个氨基酸的延伸。根据酿酒酵母遗传图谱第11版，我们的序列应该编码MAK5基因，该基因对于双链RNA杀伤质粒的维持是必需的。两个新的开放阅读框之一YBR1119预计编码一种RNA解旋酶，因此YBR1119可能对应于MAK5基因。