嗜热脂肪芽孢杆菌中苯和甲苯代谢途径的克隆与表达
Cloning and expression of a pathway for benzene and toluene from Bacillus stearothermophilus.
作者信息
Natarajan M R, Lu Z, Oriel P
机构信息
Department of Microbiology, Michigan State University, East Lansing.
出版信息
Biodegradation. 1994 Jun;5(2):77-82. doi: 10.1007/BF00700632.
PMID:7765116
Abstract
Bacillus stearothermophilus strain BR325 demonstrating broad aromatic substrate capability was isolated from petroleum-contaminated soil. The chromosomally-located aromatic pathway from this isolate was cloned into Escherichia coli as a 32 kb insert in cosmid pHC79, conferring growth on benzene, phenol, and toluene as sole carbon sources.
摘要
从石油污染土壤中分离出了具有广泛芳香底物能力的嗜热脂肪芽孢杆菌菌株BR325。该菌株位于染色体上的芳香代谢途径作为一个32 kb的插入片段克隆到黏粒载体pHC79中，并转入大肠杆菌，使后者能够以苯、苯酚和甲苯作为唯一碳源生长。
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