Hoops P, Prather N E, Berry J, Ravel J M
Infect Immun. 1976 Apr;13(4):1184-92. doi: 10.1128/iai.13.4.1184-1192.1976.
Previous work has indicated that ribosomes isolated from Salmonella typhimurium were highly immunogenic and afforded excellent protection against homologous challenge. Effective protection was obtained also when ribonucleic acid ( RNA) extracted from these ribosomes was used as a vaccine. In this investigation ribosomes prepared by another method and washed repeatedly in 1 M NH1Cl lost much of their prophylactic potency and yielded poorly protective RNA. The high-salt wash of the ribosomes was immunogenic. The RNA and the protein isolated from the salt wash of the ribosomes were effective vaccines. No intrinsic component of the ribosomes was removed by the NH4Cl wash, since the ability of both "crude" and "clean" ribosomes to function equally well in an in vitro protein synthesizing system was demonstrated. The presence of a component with toxic properties similar to those of endotoxin was found in active vaccines but not in weak ones. This was shown by the ability of effective vaccines to kill lead acetate-sensitized mice and to induce tolerance to endotoxin.
先前的研究表明，从鼠伤寒沙门氏菌中分离出的核糖体具有高度免疫原性，能为同源攻击提供出色的保护。当使用从这些核糖体中提取的核糖核酸（RNA）作为疫苗时，也能获得有效的保护。在本研究中，通过另一种方法制备并在1M氯化铵中反复洗涤的核糖体失去了大部分预防效力，产生的RNA保护效果不佳。核糖体的高盐洗涤液具有免疫原性。从核糖体的盐洗涤液中分离出的RNA和蛋白质都是有效的疫苗。氯化铵洗涤并未去除核糖体的任何内在成分，因为“粗制”和“纯净”核糖体在体外蛋白质合成系统中发挥同等作用的能力得到了证明。在活性疫苗中发现存在一种具有与内毒素类似毒性特性的成分，而在弱效疫苗中则没有。有效的疫苗能够杀死醋酸铅致敏的小鼠并诱导对内毒素的耐受性，从而证明了这一点。
