钳位均匀电场凝胶电泳显示牡蛎中存在多种创伤弧菌菌株。
Multiple Vibrio vulnificus strains in oysters as demonstrated by clamped homogeneous electric field gel electrophoresis.
作者信息
Buchrieser C, Gangar V V, Murphree R L, Tamplin M L, Kaspar C W
机构信息
Food Research Institute, University of Wisconsin, Madison 53706.
出版信息
Appl Environ Microbiol. 1995 Mar;61(3):1163-8. doi: 10.1128/aem.61.3.1163-1168.1995.
Abstract
Clamped homogeneous electric field gel electrophoresis and a computer program for managing electrophoresis banding patterns (ELBAMAP) were used to analyze genomic DNA of 118 Vibrio vulnificus strains, isolated from three oysters by direct plating. Analysis with SfiI resulted in 60 restriction endonuclease digestion profiles (REDP), while analysis with SrfI produced 53 different REDP. Similarities between REDP ranged from 7 to 93%. Principal-component analysis showed that the strains were heterogeneous.
摘要
采用钳位均匀电场凝胶电泳和一个用于管理电泳条带模式的计算机程序（ELBAMAP），对通过直接平板培养从三只牡蛎中分离出的118株创伤弧菌菌株的基因组DNA进行分析。用SfiI分析产生了60种限制性内切酶消化图谱（REDP），而用SrfI分析产生了53种不同的REDP。REDP之间的相似度在7%至93%之间。主成分分析表明这些菌株具有异质性。
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