Hutchins A M, Lampe J W, Martini M C, Campbell D R, Slavin J L
Department of Food Science and Nutrition, University of Minnesota, St Paul 55108, USA.
J Am Diet Assoc. 1995 Jul;95(7):769-74. doi: 10.1016/S0002-8223(95)00214-6.
To compare the effect of vegetable, fruit, and legume consumption on urinary isoflavonoid phytoestrogen and lignan excretion.
After 4 days of data collection, during which subjects consumed their habitual diets, subjects were randomly placed on four 9-day controlled experimental diets with each subject receiving each diet in a random order.
Seven men and three women, aged 20 to 35 years, were recruited from the University of Minnesota Twin Cities community.
All subjects consumed four experimental diets in an assigned random order: a controlled basal diet, a legume/allium diet (containing garbanzo beans, garlic, and onions), and diets low or high in vegetables and fruits (containing apples, pears, potatoes, and carrots).
Urine samples that were collected while subjects consumed their habitual diets and during the last 3 days of each feeding period were analyzed for isoflavonoid and lignan content using isotope dilution gas chromatography-mass spectrometry.
The effect of vegetable and fruit intake on urinary isoflavonoid and lignan excretion was analyzed using the general linear model procedure. Post hoc comparisons were made using Duncan's multiple range test.
Subjects excreted more of the lignan enterodiol on the high vegetable/fruit diet compared with the basal and legume/allium diets (P = .03); more of the isoflavonoids O-desmethylangolensin (O-DMA), genistein, and sum of isoflavonoids on the legume/allium diet compared with the other controlled diets (P < .05); and more of the isoflavan equol on the basal and legume/allium diets compared with the high vegetable/fruit diet (P < .01). Subjects who excreted higher levels of equol on the basal and legume/allium diets also consumed more of the milk-based pudding provided as part of the controlled diets.
Urinary lignan and isoflavonoid excretion changed in response to alterations in vegetable, fruit, and legume intake under controlled dietary conditions.
比较蔬菜、水果和豆类摄入对尿中异黄酮类植物雌激素和木脂素排泄的影响。
在收集4天数据期间，受试者按习惯饮食，之后将受试者随机安排接受四种为期9天的对照实验饮食，每位受试者按随机顺序接受每种饮食。
从明尼苏达大学双城分校社区招募了7名男性和3名女性，年龄在20至35岁之间。
所有受试者按指定随机顺序食用四种实验饮食：对照基础饮食、豆类/葱属植物饮食（含鹰嘴豆、大蒜和洋葱）以及蔬菜和水果含量低或高的饮食（含苹果、梨、土豆和胡萝卜）。
收集受试者按习惯饮食时以及每个喂食期最后3天的尿液样本，采用同位素稀释气相色谱 - 质谱法分析异黄酮和木脂素含量。
采用一般线性模型程序分析蔬菜和水果摄入量对尿中异黄酮和木脂素排泄的影响。采用邓肯多重极差检验进行事后比较。
与基础饮食和豆类/葱属植物饮食相比，受试者在高蔬菜/水果饮食中排泄的木脂素肠二醇更多（P = 0.03）；与其他对照饮食相比，豆类/葱属植物饮食中排泄的异黄酮O - 去甲基安哥拉ensin（O - DMA）、染料木黄酮和异黄酮总量更多（P < 0.05）；与高蔬菜/水果饮食相比，基础饮食和豆类/葱属植物饮食中排泄的异黄烷雌马酚更多（P < 0.01）。在基础饮食和豆类/葱属植物饮食中排泄雌马酚水平较高的受试者，食用作为对照饮食一部分提供的乳基布丁也更多。
在可控饮食条件下，尿中木脂素和异黄酮排泄随蔬菜、水果和豆类摄入量的变化而改变。
