两株牛肠道病毒5'非编码区和衣壳编码基因组区域的核苷酸序列。

The nucleotide sequence of the 5'non-coding and capsid coding genome regions of two bovine enterovirus strains.

作者信息

McNally R M, Earle J A, McIlhatton M, Hoey E M, Martin S J

机构信息

Division of Molecular Biology, School of Biology and Biochemistry, Queen's University of Belfast, Northern Ireland.

出版信息

Arch Virol. 1994;139(3-4):287-99. doi: 10.1007/BF01310792.

DOI:10.1007/BF01310792

PMID:7832636

Abstract

The sequence of cDNA clones representing the 5' non-coding regions (NCR) and capsid regions of two bovine enteroviruses (strains PS-87 and RM-2; serotype two viruses) have been determined and compared with that obtained from a serotype one strain (VG-5-27). All three strains showed a longer 5' NCR compared to human enteroviruses and rhinoviruses due in part to a hundred residue insertion approximately at a hundred residues in from the 5' end. However, another domain occurring at nucleotide 187-222 in poliovirus is absent in each bovine enterovirus. Comparisons of the predicted structural protein amino acid sequences indicate that PS-87 shares most sequence identity with RM-2 and then with VG-5-27 in that order. The VP1 protein of PS-87 and RM-2 are shorter than the equivalent VP1 of VG-5-27 due in part to a truncation at their C-terminii. VP3 is only slightly smaller than VP2 in each virus.

摘要

已确定了代表两种牛肠道病毒（PS - 87株和RM - 2株；血清型2病毒）5'非编码区（NCR）和衣壳区的cDNA克隆序列，并与从血清型1株（VG - 5 - 27）获得的序列进行了比较。与人类肠道病毒和鼻病毒相比，所有这三株病毒的5' NCR都更长，部分原因是在距5'端约一百个残基处有一个一百个残基的插入。然而，脊髓灰质炎病毒中位于核苷酸187 - 222处的另一个结构域在每种牛肠道病毒中都不存在。对预测的结构蛋白氨基酸序列的比较表明，PS - 87与RM - 2的序列一致性最高其次是与VG - 5 - 27。PS - 87和RM - 2的VP1蛋白比VG - 5 - 27的相应VP1蛋白短，部分原因是其C末端有截短。在每种病毒中，VP3仅比VP2略小。