Birch M, Nolard N, Shankland G S, Denning D W
University of Manchester Department of Medicine, Hope Hospital, Salford.
Epidemiol Infect. 1995 Feb;114(1):161-8. doi: 10.1017/s0950268800052018.
Invasive aspergillosis is often nosocomially acquired and carries a high mortality. Molecular typing methods to discriminate isolates have now been developed. Using simple restriction endonuclease (Sal1 and Xho1) digestion of total genomic DNA, we have typed 25 epidemiologically-related isolates of A. fumigatus from six hospital episodes of invasive aspergillosis. Eight DNA types were found and in each case the DNA type matched precisely the epidemiological data. Thus DNA typing of A. fumigatus can provide the means to match isolates from linked sources and distinguish isolates from diverse origins.
侵袭性曲霉病常通过医院感染获得，且死亡率很高。目前已开发出用于区分分离株的分子分型方法。通过对总基因组DNA进行简单的限制性内切酶（Sal1和Xho1）消化，我们对来自6例侵袭性曲霉病医院感染病例的25株具有流行病学关联的烟曲霉分离株进行了分型。发现了8种DNA类型，并且在每种情况下，DNA类型都与流行病学数据精确匹配。因此，烟曲霉的DNA分型可为匹配来自相关来源的分离株以及区分来自不同来源的分离株提供方法。
