Hobson R P, Williams A, Rawal K, Pennington T H, Forbes K J
Department of Medical Microbiology, University of Aberdeen, Foresterhill.
Epidemiol Infect. 1995 Feb;114(1):93-103. doi: 10.1017/s0950268800051943.
A PCR-based method of detecting Haemophilus influenzae in cultures inoculated from throat swabs was evaluated using samples from groups of laboratory staff and medical students and then applied to samples originating from the closed human community of an Antarctic research station. Suitable PCR primers to an H. influenzae gene (ompP2) were used to amplify the gene from DNA preparations made from mixed growth on chocolate agar with added vancomycin. PCR product was reamplified and subjected to restriction endonuclease digestion to allow temporal and spatial mapping of strains over an 8-month period. Eleven different strains of H. influenzae were detected. One particular strain was detected in a third of the base members.
一种基于聚合酶链反应（PCR）的方法用于检测从咽喉拭子接种的培养物中的流感嗜血杆菌，该方法首先使用来自实验室工作人员和医学生群体的样本进行评估，然后应用于源自南极研究站封闭人类社区的样本。针对流感嗜血杆菌基因（ompP2）的合适PCR引物用于从添加了万古霉素的巧克力琼脂上的混合生长物制备的DNA中扩增该基因。PCR产物进行再次扩增，并进行限制性内切酶消化，以便在8个月的时间内对菌株进行时间和空间定位。共检测到11种不同的流感嗜血杆菌菌株。在三分之一的基地成员中检测到一种特定菌株。
