Garcia O F, Ramalho A T, Di Giorgio M, Mir S S, Espinoza M E, Manzano J, Nasazzi N, López I
Secretaria Ejecutiva para Asuntos Nucleares (SEAN-CPHR), Playa, Ciudad Habana, Cuba.
Mutat Res. 1995 Mar;327(1-2):33-9. doi: 10.1016/0027-5107(94)00066-e.
As part of a regional International Atomic Energy Agency (IAEA) collaborative project within Latin America, five countries participated in an intercomparison in cytogenetic dosimetry. Coded slides for chromosomal aberrations and micronucleus analyses were prepared by the coordinator laboratory which organized the exercise and sent to the other participating laboratories. For estimates of dose, each laboratory scored the frequency of dicentrics in metaphases and the frequency of micronuclei in binucleated cells. The lymphocytes were irradiated with 60Co gamma-rays (0, 0.75, 1.5 and 3.0 Gy). Eleven of the 15 estimates of dose based on dicentrics and nine of the 12 based on micronuclei fell within +/- 30% of the true dose. When considering the uncertainties of the dose estimates, the true dose fell within the 95% confidence limits of the estimates on eight of the 15 occasions for dicentrics and four of the 12 for micronuclei.
作为拉丁美洲地区国际原子能机构（IAEA）合作项目的一部分，五个国家参与了细胞遗传学剂量测定的比对。用于染色体畸变和微核分析的编码玻片由组织此次活动的协调实验室制备，并发送给其他参与实验室。为了估计剂量，每个实验室对中期双着丝粒的频率和双核细胞中微核的频率进行评分。淋巴细胞用60Coγ射线（0、0.75、1.5和3.0 Gy）照射。基于双着丝粒的15个剂量估计中有11个，基于微核的12个剂量估计中有9个落在真实剂量的±30%范围内。考虑到剂量估计的不确定性，在基于双着丝粒的15次估计中，有8次真实剂量落在估计值的95%置信区间内；在基于微核的12次估计中，有4次真实剂量落在估计值的95%置信区间内。
