Evdokimov Y M, Pyatigorskaya T L, Polyvtsev O F, Akimenko N M, Kadykov V A, Tsvankin D Y, Varshavsky Y M
Nucleic Acids Res. 1976 Sep;3(9):2353-66. doi: 10.1093/nar/3.9.2353.
Comparative CD and X-ray diffraction studies of DNA compact particules which were obtained in PEG-containing water-salt solutions, have been carried out. Compact particles, formed from native DNA, produce a psi CD spectrum (characterized by a negative band at lambda-270 nm) and a small-angle X-ray diffraction pattern, which shows two reflections: I at 34-40 A and II at 80-90 A (together with its second-order reflection). Compact particules, formed from DNA molecules with partially disordered secondary structure, do not produce the psi CD spectrum and the reflection I, while the reflection II remains unchanged. It is suggested that the spacing of 34-40 A is associated with a side-by-side packing of DNA fragments in "microcrystallization' regions in compact particules and that such "microcrystallization' accounts for the generation of the psi CD spectrum.
对在含聚乙二醇的水盐溶液中获得的DNA紧密颗粒进行了圆二色性（CD）和X射线衍射对比研究。由天然DNA形成的紧密颗粒产生一个ψ CD光谱（其特征是在λ-270nm处有一个负峰）和一个小角X射线衍射图谱，该图谱显示出两个反射峰：一个在34-40 Å处的I峰和一个在80-90 Å处的II峰（及其二级反射峰）。由具有部分无序二级结构的DNA分子形成的紧密颗粒不会产生ψ CD光谱和I反射峰，而II反射峰保持不变。有人提出，34-40 Å的间距与紧密颗粒中“微晶化”区域内DNA片段的并排堆积有关，并且这种“微晶化”解释了ψ CD光谱的产生。
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