Fauci A S, Pratt K R
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3676-9. doi: 10.1073/pnas.73.10.3676.
A culture and assay system for the stimulation of human peripheral blood lymphocytes with polyclonal activators of bone-marrow-derived lymphocytes (B cells), such as pokeweed mitogen and Escherichia coli lipopolysaccharide, and subsequent measurement of single cell antibody production by a hemolysis-in-bel direct plaque-forming cell assay against sheep erythrocytes has been established. The critical culture requirements have been delineated and a new highly sensitive ultrathin gel assay method has been described. Under these conditions a substantial and highly reproducible plaque-forming cell response was detected in normal human peripheral blood. This system can be readily used to explore the complex events associated with activation of human B cells.
已建立一种培养和检测系统，该系统使用骨髓衍生淋巴细胞（B细胞）的多克隆激活剂（如商陆有丝分裂原和大肠杆菌脂多糖）刺激人外周血淋巴细胞，随后通过针对绵羊红细胞的溶血直接空斑形成细胞检测法测量单细胞抗体产生。已明确了关键的培养条件，并描述了一种新的高灵敏度超薄凝胶检测方法。在这些条件下，在正常人外周血中检测到了大量且高度可重复的空斑形成细胞反应。该系统可轻松用于探索与人类B细胞激活相关的复杂事件。
