Ohshima S, Shimizu Y, Takahama M
Second Department of Pathology, Saitama Medical School, Japan.
Virchows Arch. 1994;424(2):129-34. doi: 10.1007/BF00193491.
DNA of minute specimens taken from formalin-fixed and paraffin-embedded tissue was used as a template for the polymerase chain reaction (PCR) to examine whether the c-Ki-ras gene is activated in atypical bronchioloalveolar cell hyperplasia (ABH) of human lung. The c-Ki-ras gene was successfully amplified on 131 samples from 29 cases of lung adenocarcinoma (87.3% of all samples used as templates) by the nested PCR method. Point mutations at codon 12 of the c-Ki-ras gene could be detected in carcinoma tissue of 6 cases (20.7% of all cases), also detected in ABHs showing moderate to severe atypia of 2 cases. PCR amplification is a useful technique for studying pin-point pathological lesions in a routine paraffin section at the molecular level.
取自福尔马林固定石蜡包埋组织的微小标本的DNA被用作聚合酶链反应（PCR）的模板，以检测人肺非典型细支气管肺泡细胞增生（ABH）中c-Ki-ras基因是否被激活。通过巢式PCR方法，在29例肺腺癌的131个样本（占所有用作模板样本的87.3%）上成功扩增出c-Ki-ras基因。在6例癌组织（占所有病例的20.7%）中可检测到c-Ki-ras基因第12密码子的点突变，在2例显示中度至重度异型性的ABH中也检测到该突变。PCR扩增是在分子水平研究常规石蜡切片中微小病理病变的有用技术。
