用于鉴定肠杆菌科细菌的迷你泰克鉴别系统的临床评估
Clinical evaluation of the minitek differential system for identification of Enterobacteriaceae.
作者信息
Finklea P J, Cole M S, Sodeman T M
出版信息
J Clin Microbiol. 1976 Nov;4(5):400-4. doi: 10.1128/jcm.4.5.400-404.1976.
Forty-one stock organisms and 581 fresh clinical isolates were used in comparing the Minitek system to conventional tubed media to determine if this system is feasible and accurate for a high-volume clinical microbiology laboratory. In addition to comparison with tubed media, the following parameters were tested: (i) reproducibility of disks, (ii) the effect of variation in inoculum size, (iii) the effect of the age of culture, and (iv) the effect of predispensing disks. A total of 5,947 disks were compared with tube reactions; 95.9% agreed on the initial reading. After repeating questionable tube and disk reactions, this agreement rose to 98.2%. Using the disk reactions and the identification schema currently in use with tubed media, 94.9% of the Minitek final identifications agreed with those of tubed media; 1.5% gave differing identifications and 3.6% were inconclusive. This system is accurate, inexpensive, flexible, and convenient.
使用41种标准菌株和581份新鲜临床分离株，将Minitek系统与传统的试管培养基进行比较，以确定该系统对于大容量临床微生物实验室是否可行且准确。除了与试管培养基进行比较外，还测试了以下参数：（i）药敏纸片的重复性，（ii）接种量变化的影响，（iii）培养物菌龄的影响，以及（iv）预分发药敏纸片的影响。总共将5947张药敏纸片与试管反应进行了比较；初次读数时95.9%一致。在重复有疑问的试管和药敏纸片反应后，这一一致性上升至98.2%。使用当前与试管培养基一起使用的药敏纸片反应和鉴定模式，Minitek最终鉴定结果中有94.9%与试管培养基的鉴定结果一致；1.5%给出了不同的鉴定结果，3.6%为不确定结果。该系统准确、廉价、灵活且方便。
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